FastQCFastQC Report
Sat 14 Apr 2018
HLTFNBGX5_n01_mdex_gr_dam_250_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFNBGX5_n01_mdex_gr_dam_250_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30772440
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGAGACAAGGGTTCACATTTCG676460.21982657208853115No Hit
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTGAGTGTCGCCCATGGGCATCGACACC615050.1998704035169132No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG508350.1651965200029637No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCGACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG377620.12271370096098977No Hit
GGTCGCGGCCGAGGAACTAACAGAACTCGCCGTGAAGACTGGCGAACAGTTCATACAGAGTCTTTTACGACTCAA354900.11533047103187137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG4543050.068.3792341
GTCGCGG4658000.066.6635062
CGCGGCC4676950.066.5849844
TCGCGGC4668050.066.5338063
CGGCCGA4682300.066.4682856
AGGATCT1117150.065.6386312
AGGATCA1034500.064.5139912
GGATCTA210450.064.12034613
GCCGAGG4866000.063.829928
GCGGCCG4884750.063.648655
GGCCGAG4891350.063.5063677
GGATCTT510850.063.14235313
GGATCCA657550.062.31524713
GGATCTC249950.062.2495413
GGATCAT468050.062.11747713
GAGGATC4551850.061.2145811
GGATCAA384350.060.8979813
AGGATCC1696950.060.62121212
GGATCAC120000.060.161613
GGATCTG203100.059.90535713
Produced by FastQC (version 0.11.2)