FastQCFastQC Report
Sat 14 Apr 2018
HLTFNBGX5_n01_mdex_gr_dam_200_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFNBGX5_n01_mdex_gr_dam_200_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29311684
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGAGACAAGGGTTCACATTTCG685810.23397154527184447No Hit
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTGAGTGTCGCCCATGGGCATCGACACC597100.2037071633277706No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG506480.17279116409688366No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCGACGAAGCACGCCCATCCAACCTAGGCGGGTGGAATCGATGCCCATGG377930.1289349325681868No Hit
GGTCGCGGCCGAGGAACTAACAGAACTCGCCGTGAAGACTGGCGAACAGTTCATACAGAGTCTTTTACGACTCAA314690.10735991831789672No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG4607300.068.4315641
GTCGCGG4743150.066.407732
CGCGGCC4768150.066.296024
TCGCGGC4764050.066.212673
CGGCCGA4775450.066.119866
AGGATCT1187200.065.1767612
AGGATCA1051450.064.2335912
GGATCTA223050.063.8203313
GGCCGAG4957550.063.415667
GCGGCCG4975600.063.3170785
GCCGAGG4981850.063.1402448
GGATCTT552300.062.87821213
GGATCAT469700.062.3312513
GGATCTC264200.061.93145413
GGATCCA704050.061.48429513
GGATCGG159050.060.43318613
GGATCAA392300.060.40982413
GGATCAC124500.060.09063313
GAGGATC4774200.059.66676311
AGGATCC1759100.059.63472712
Produced by FastQC (version 0.11.2)