Basic Statistics
Measure | Value |
---|---|
Filename | HLTFNBGX5_n01_gdna.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 14419451 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 58 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 164987 | 1.1441975148707118 | No Hit |
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCAGATCGGAAGAGCACAC | 25451 | 0.17650463946234846 | No Hit |
ACCCTTATTCCACTGCACCGTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCG | 20638 | 0.14312611485693874 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG | 18627 | 0.12917967542592296 | RNA PCR Primer, Index 29 (100% over 43bp) |
TCCTCGGCCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGCCGTCTTCTG | 15059 | 0.10443532142797947 | RNA PCR Primer, Index 29 (100% over 39bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CACCGTC | 32070 | 0.0 | 46.912285 | 16 |
ACCGTCC | 32560 | 0.0 | 46.16427 | 17 |
CCGTCCT | 32290 | 0.0 | 46.15556 | 18 |
ATGCGGC | 3490 | 0.0 | 42.37651 | 15 |
GTATGCG | 3545 | 0.0 | 42.302383 | 13 |
TATGCGG | 3505 | 0.0 | 42.096794 | 14 |
GATAAGT | 3670 | 0.0 | 41.70741 | 8 |
CGTCCTC | 36160 | 0.0 | 41.27486 | 19 |
ATAAGTA | 3790 | 0.0 | 40.022873 | 9 |
TAAGTAT | 3765 | 0.0 | 40.013935 | 10 |
ACCGAAG | 4135 | 0.0 | 39.19155 | 2 |
AGTATGC | 3835 | 0.0 | 39.013615 | 12 |
CACCGAT | 7735 | 0.0 | 38.50829 | 16 |
CACCGAA | 4245 | 0.0 | 38.354935 | 1 |
ACCCTTA | 69750 | 0.0 | 38.288284 | 1 |
AAGTATG | 3930 | 0.0 | 38.158253 | 11 |
TGCACCG | 67590 | 0.0 | 37.947556 | 14 |
CCCTTAT | 71595 | 0.0 | 37.430035 | 2 |
CTGCACC | 69115 | 0.0 | 37.4094 | 13 |
CCTTATT | 71320 | 0.0 | 37.380714 | 3 |