FastQCFastQC Report
Sat 14 Apr 2018
HLTFNBGX5_n01_gdna.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFNBGX5_n01_gdna.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14419451
Sequences flagged as poor quality0
Sequence length75
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1649871.1441975148707118No Hit
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCGACCAGATCGGAAGAGCACAC254510.17650463946234846No Hit
ACCCTTATTCCACTGCACCGTCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGATCCTCGGCCGCG206380.14312611485693874No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG186270.12917967542592296RNA PCR Primer, Index 29 (100% over 43bp)
TCCTCGGCCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGCCGTCTTCTG150590.10443532142797947RNA PCR Primer, Index 29 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGTC320700.046.91228516
ACCGTCC325600.046.1642717
CCGTCCT322900.046.1555618
ATGCGGC34900.042.3765115
GTATGCG35450.042.30238313
TATGCGG35050.042.09679414
GATAAGT36700.041.707418
CGTCCTC361600.041.2748619
ATAAGTA37900.040.0228739
TAAGTAT37650.040.01393510
ACCGAAG41350.039.191552
AGTATGC38350.039.01361512
CACCGAT77350.038.5082916
CACCGAA42450.038.3549351
ACCCTTA697500.038.2882841
AAGTATG39300.038.15825311
TGCACCG675900.037.94755614
CCCTTAT715950.037.4300352
CTGCACC691150.037.409413
CCTTATT713200.037.3807143