FastQCFastQC Report
Sat 14 Apr 2018
HLTFNBGX5_n01_gdna_200.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFNBGX5_n01_gdna_200.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53051427
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1547320.29166416202150414No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGTC246850.028.60368516
ACCGTCC268250.026.37324517
CCGTCCT274150.025.5292618
CCGGATG124600.023.90033769
CGTCTTG54100.023.47263769
CGTCCTC317150.022.19916519
ATCGGTG72200.020.53297615
TGCCGTG68950.020.46917569
TGGGGGG61850.019.9656661
TCTCGTT63600.019.53244869
ATGCCGG106250.018.67455369
TTCTGCT85100.017.96327269
ACCCTTA775700.016.8889121
CCCTTAT800950.016.4191992
CCTTATT802200.016.333413
TTATTCC807950.016.1019255
GTATGCG24650.016.08426313
TCGGTGC80500.016.06055516
TGCACCG795200.015.99384614
TATTCCA812050.015.9610926
Produced by FastQC (version 0.11.2)