FastQCFastQC Report
Sat 3 Dec 2022
HLTFMDRX2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFMDRX2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1881433919
Sequences flagged as poor quality0
Sequence length10
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAGAACGT57135212630.3679082337199No Hit
AGGGCACGTG41627985022.125669458604037No Hit
TTAGGGTCGT40515707021.53448313589163No Hit
AGAATACAGG38162718920.283847609319093No Hit
AGATCTCGGT504656262.682295960031536Illumina Single End PCR Primer 1 (100% over 10bp)
GGGGGGGGGG47142570.2505672377005764No Hit
GTAGGGTCGT39246570.2085992476464968No Hit
ACGACAACTT19953950.1060571397086628No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)