FastQCFastQC Report
Tue 2 Jul 2019
HLTFCAFXY_n02_vk1-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFCAFXY_n02_vk1-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11448353
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG332630.29054834350408304No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG182260.1592019393531978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC132650.028.17397355
CGCCGTA132400.028.04265453
GCCGTAT141950.027.2157154
CGTATCA140200.027.0561156
TCGCCGT137250.026.9498452
GTCGCCG142150.026.56233851
GGTCGCC149300.026.27456950
TGGTCGC156550.024.7462349
TGTCGGC41950.024.27696830
GTATCAT151400.023.6430557
GTGGTCG169800.023.1040548
CTCGGTG155150.022.75953344
GTCGGCG44700.022.54873831
CGGCGGC45950.021.85926433
ATCTCGG163850.021.52951642
TCTCGGT163650.021.4918643
GTGGGAC48250.021.25182219
GGTGGTC181950.021.13809447
GATCTCG165750.021.02733241
TCATTAA186350.020.74967460
Produced by FastQC (version 0.11.5)