FastQCFastQC Report
Tue 2 Jul 2019
HLTFCAFXY_n02_vk1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFCAFXY_n02_vk1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13276878
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG266620.20081528202639207No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG213560.1608510675476569No Hit
CGGCAAAATGTTAGTGCAGGAAAGCGGGAAGGAATAAGAAGCAACTAAAC135070.10173325385681785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAG36950.020.3078521
CGGGCTA7400.019.4274941
CGGCAAA123050.019.0922811
TGTCGGC37400.018.807930
CGGGAAG159500.018.5323941
AAGCGGG73600.018.25826322
GTCGGCG40200.017.84618231
CGCGAAT18150.017.7736191
CGCCAAG33950.017.7645131
GTGCAGG75450.017.53367414
CGGCGGC40900.017.3696833
ACGCGGT33750.017.21229237
CGCGGTA34950.017.12246938
CGGGCAG14350.017.1045171
AGCGGGA80100.016.86388223
TTAGTGC79800.016.79850611
TCGGCGG42100.016.7082732
GTGGGAC42900.016.31530219
TTAACCG113850.016.22901558
CGCCAAA53850.015.8879821
Produced by FastQC (version 0.11.5)