FastQCFastQC Report
Tue 2 Jul 2019
HLTFCAFXY_n02_vk1-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFCAFXY_n02_vk1-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7616753
Sequences flagged as poor quality0
Sequence length76
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG195900.25719620946090804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC138750.036.97328655
CGCCGTA140950.036.64470353
TCGCCGT140750.036.24901652
CGTATCA143400.036.16456656
GTCGCCG145050.035.8257951
GGTCGCC150700.035.1100450
GCCGTAT152250.034.4535754
TGGTCGC153100.034.357149
GTGGTCG160050.032.99659748
CTCGGTG148500.032.99373244
ATCTCGG151350.032.18661542
TCTCGGT151600.032.1805543
GATCTCG151000.031.98016241
GGTGGTC167850.031.40069247
TCGGTGG167200.031.04113845
AGATCTC158650.030.90088540
CGAGATC17450.030.5114965
CGGTGGT175550.030.0634646
TAGATCT177700.028.5367939
GTATCAT185700.026.8887557
Produced by FastQC (version 0.11.5)