FastQCFastQC Report
Tue 2 Jul 2019
HLTFCAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFCAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17273975
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG475971227.554236937358077No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN570460.33024246011702574No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG458460.2654050385044554No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC427610.24754580228349296Illumina Single End PCR Primer 1 (100% over 50bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG202160.11703154601068949No Hit
GGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG186050.10770537759838138No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG174390.10095533888407272No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG382900.065.2576618
CACACGA249450.064.9229119
ACACGAG252000.064.8175620
AGCACAC381900.063.82467317
AGAGCAC526050.063.7362615
CACGTAG131550.062.75863621
GAGCACA503050.062.1674316
CACGAGA276050.060.6385821
ACGAGAT278400.059.82502422
GCACGCG61600.059.03564518
TAACTGA173200.058.9855232
AAGAGCA674150.058.8843214
ACACGTA129550.057.9759320
CACACGT135050.057.92485419
ACGTAGA138400.056.51683422
TCATACT97050.056.08360312
CACGCGA33750.055.0684419
CATACTT98950.054.37013213
ACTGAAG183550.054.1400074
ATAACTG194500.053.9024771
Produced by FastQC (version 0.11.5)