FastQCFastQC Report
Tue 2 Jul 2019
HLTFCAFXY_n02_ncm2-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFCAFXY_n02_ncm2-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14197727
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2830281.9934740258070887Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2436221.715922555772484No Hit
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT167090.11768785242877258Illumina Single End PCR Primer 1 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGAGA548300.054.02801515
GAGCAAG315750.053.89082316
GCACAAG237600.053.27673718
AAGAGCA755250.053.01106614
AGAGCAC407150.052.72916415
GAGCACA401300.052.57112516
AGATAGA396550.052.5286147
GAGAGAT566600.051.92872616
GATCGAG118450.050.7534188
AGAGCAA345950.050.2608515
AGCACAA254750.049.92434717
GATAGAT415850.049.6926968
AAGAGAG607400.049.30207414
GAAGAGA682350.048.805913
ACACTCT99450.048.8031851
GCAGATC331350.048.3018155
AGCAAGA357100.047.9052417
GGCAGAT160350.047.5897254
CAGATAG147850.047.1357776
ACACGAG96150.047.09763320
Produced by FastQC (version 0.11.5)