FastQCFastQC Report
Tue 2 Jul 2019
HLTFCAFXY_n02_ncm2-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFCAFXY_n02_ncm2-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15278920
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2221121.4537153149568165No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2170871.420826864726041Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACAAG225350.052.7235218
GAGCAAG313100.052.4438116
AGAGCAC338500.051.7884615
AAGAGCA686550.051.67061214
GAGCACA333600.051.59192716
AGATAGA409900.050.8539777
GATCGAG117650.049.7311748
GAGAGAT573850.049.72082516
AGAGAGA586800.049.11515
GATAGAT421500.048.9368068
AGAGCAA343300.048.80117415
AGCACAA242250.048.45292717
AAGAGAG624000.046.8441214
GCAGATC298050.046.5307355
CAGATAG155250.045.8383336
AGCAAGA361100.045.58904617
GAAGAGA715900.045.5294213
ACACTCT102000.044.1407971
AGATCGA139800.043.9328547
CACAAGA271350.043.5404919
Produced by FastQC (version 0.11.5)