FastQCFastQC Report
Tue 2 Jul 2019
HLTFCAFXY_n02_ncm2-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFCAFXY_n02_ncm2-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13301362
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2569091.931448824563981No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC962570.7236627346883726Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC138400.043.74608215
GAGCACA134500.043.737616
GATCGAG56350.041.4466978
GCACAAG96450.041.0004918
AGATAGA207450.040.9166537
AGAGAGA224150.040.89082315
GAGCAAG126100.040.4069316
AAGAGCA296250.039.76487414
GATAGAT213400.039.675278
GAGAGAT231250.039.5276516
GCAGATC146700.037.607295
CAGATAG84650.035.2493256
AAGAGAG269500.034.6863814
ATAGATC244500.034.6650779
AGAGATC264350.034.4991117
AGAGCAA152250.034.4107115
AGCACAA117700.033.92516717
AGATCGA73900.032.837477
GAAGAGA334150.031.44244813
ACACTCT54900.031.4231511
Produced by FastQC (version 0.11.5)