FastQCFastQC Report
Tue 2 Jul 2019
HLTFCAFXY_n02_ncm2-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFCAFXY_n02_ncm2-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13750583
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3165922.3023896514060533No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC570580.414949678860889Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA118550.040.26548416
AGAGCAC121400.040.0132215
AGAGAGA195400.037.21805615
GAGAGAT198650.036.37906616
GCACAAG91900.036.2143218
AAGAGCA262100.035.9853814
GAGCAAG115350.035.4663916
AGAGATC217350.033.26524717
GCAGATC123800.032.483685
GATCGAG39750.031.7983938
AGATAGA157000.031.4242887
AAGAGAG233400.031.39858814
GATAGAT156800.031.2620248
AGCACAA113500.029.7235917
CAGATCG282400.029.4897356
CGGGGAC19200.029.2237641
AGAGCAA145150.029.2225615
GAAGAGA292850.028.23963713
ATAGATC176800.027.7797499
GCAAGAT161200.025.4435618
Produced by FastQC (version 0.11.5)