FastQCFastQC Report
Tue 2 Jul 2019
HLTFCAFXY_n02_ncm2-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFCAFXY_n02_ncm2-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18089306
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2937461.6238655037401655No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1648010.9110410316459902Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA327900.050.2157616
AGAGCAC337350.050.13930515
GAGCAAG274750.049.2813816
AAGAGCA659500.048.07969314
GATCGAG109250.047.883188
AGATAGA374550.047.624397
GCACAAG208650.047.4163418
AGAGAGA484950.047.32062515
GAGAGAT496650.045.9077316
GATAGAT387850.045.6273578
AGAGCAA313350.044.10620515
AGCACAA231000.042.72284317
AAGAGAG543050.042.59358214
GCAGATC300500.042.5256165
CAGATAG146550.041.9164246
AGATCGA133750.041.208927
CACACGA83950.040.89476819
AGAGATC561950.040.5047417
GGCAGAT158000.040.1150364
ACACGAG87200.040.0107320
Produced by FastQC (version 0.11.5)