FastQCFastQC Report
Tue 2 Jul 2019
HLTFCAFXY_n02_ncm2-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFCAFXY_n02_ncm2-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13757834
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2787502.026118355549282Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2274871.6535088299509937No Hit
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT167760.12193779922042962Illumina Single End PCR Primer 1 (100% over 39bp)
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC138700.10081528822051494Illumina Single End PCR Primer 1 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAG310800.054.70129816
GCACAAG221100.054.14877318
AGAGAGA553400.054.05294415
AGATAGA418050.053.917647
GATCGAG112300.053.7848138
AAGAGCA695800.051.7492514
AGAGCAC341100.051.26082215
AGAGCAA338300.051.24024215
GAGAGAT580150.051.22604416
GATAGAT436450.051.192298
GAGCACA339050.050.8660616
AGCACAA240100.050.17005517
CAGATAG154250.049.452226
AAGAGAG612400.049.2354114
GAAGAGA703850.047.8907813
AGCAAGA358900.047.76075417
GCAGATC319650.047.2785875
ACACTCT104600.047.039531
GGCAGAT157450.046.84
ATCGAGA132000.045.9871989
Produced by FastQC (version 0.11.5)