FastQCFastQC Report
Tue 2 Jul 2019
HLTFCAFXY_n02_ncm2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFCAFXY_n02_ncm2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14393803
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2666571.8525819757294164Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2530511.7580551852766084No Hit
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT178180.12378938352845317Illumina Single End PCR Primer 1 (100% over 39bp)
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC150040.10423930353916891Illumina Single End PCR Primer 1 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATAGA493300.055.1812177
GATCGAG136400.054.959028
GAGCAAG331750.054.67584216
AGAGAGA581900.052.90881315
GATAGAT513650.052.60398
AAGAGCA762500.052.4218214
GCACAAG243750.052.31948518
CAGATAG176450.052.019566
GAGCACA386000.051.5337716
AGAGCAC395150.051.46721615
AGAGCAA361850.051.12582815
GAGAGAT607000.050.40771516
ACACTCT107300.049.840551
AGCACAA257450.049.522117
AGATCGA161150.049.0627067
GCAGATC333850.048.6467635
GGCAGAT168750.048.357774
AGCAAGA378300.048.18931217
ATCGAGA156350.048.1836249
AAGAGAG649650.047.84969314
Produced by FastQC (version 0.11.5)