FastQCFastQC Report
Tue 2 Jul 2019
HLTFCAFXY_n02_ncm2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFCAFXY_n02_ncm2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13764140
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2845352.0672196010793265No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC492230.3576176935137248Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC102800.037.7873815
GAGCACA101650.037.28424516
AAGAGCA226600.032.85001414
GATCGAG40100.030.9945778
GAGAGAT172600.030.93983516
AGAGAGA176000.030.78046815
GATAGAT154350.030.7350858
GAGCAAG101800.030.69785116
AGATAGA154700.030.6444767
GCACAAG75900.029.96881518
GCACACG36450.029.1859618
AGAGATC189000.028.95839517
GCAGATC112750.028.022695
ATAGATC177150.026.8921479
CGGGGAC15000.026.8858091
ACACGAG30750.026.28824820
GTGACTG47300.026.1715891
CAGATCG268100.025.5222856
CAGATAG67650.024.950036
AGAGCAA131700.024.87178815
Produced by FastQC (version 0.11.5)