FastQCFastQC Report
Tue 2 Jul 2019
HLTFCAFXY_n01_vk1-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFCAFXY_n01_vk1-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11448353
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCACTCTTCTTCTGAAGAGTTAAGCACTCCATTATGCTCATTGGGTTG136220.11898654767196644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACA41000.029.4528711
GCGTGAA27850.023.75181266
GCCGTCT45050.023.22749556
TATGCCG63750.022.34722953
AATCGCG46450.022.2368346
GCCGGCT16550.021.35744756
TGCGTGA23950.021.18959265
GTATGCC73350.021.04798552
TGCCGTC60500.020.76830755
TGCCGGC24900.020.66236555
GGGATTC38300.019.7368232
ATCGCGT33550.019.51576847
ATGCCGT63350.019.50360154
GTCACAC233000.018.76064336
CACTTGA232250.018.64243140
GTTGGGA42650.018.62624229
ATGCCGG24800.018.48869754
CCGTCTT69500.018.42970857
ACACTTG239650.018.10965239
AATCTCG106150.018.07578546
Produced by FastQC (version 0.11.5)