FastQCFastQC Report
Tue 2 Jul 2019
HLTFCAFXY_n01_vk1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFCAFXY_n01_vk1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13276878
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCACTCTTCTTCTGAAGAGTTAAGCACTCCATTATGCTCATTGGGTTG142420.10726919385717033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACA27300.020.8982491
CCTATGC46200.017.04348412
GGCTTCC51400.015.5915837
CTTTCCC305950.014.5960192
TCTTTCC331400.013.8569051
CCGGGCT8600.013.83607925
CTCTTTC283450.013.0892371
CCCTACA355100.013.08820156
CGGGCTG9550.012.82603326
TCCCTAC376250.012.5292095
CCTACAC387800.012.1285627
TTCCCTA396500.011.9687584
CTTGGCT73500.011.5704844
GGCCGCC10600.011.55583517
CTACACG408300.011.5538938
GGGATTC41900.011.526113532
CTATGCT72000.011.42229113
ACACGAC410050.011.34242710
ACACTCT63850.011.292531
TACACGA416250.011.2575589
Produced by FastQC (version 0.11.5)