FastQCFastQC Report
Tue 2 Jul 2019
HLTFCAFXY_n01_vk1-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFCAFXY_n01_vk1-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7616753
Sequences flagged as poor quality0
Sequence length76
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGAA22000.041.68095466
TGCGTGA20900.038.34848865
CTGCGTG19050.037.4765964
GCCGTCT88300.037.2947856
TGCCGTC98550.036.221955
TATGCCG106100.035.4645953
CTGCTTG83450.035.18523464
CGTGAAA28050.035.063567
GCCCACA20300.034.827091
ATGCCGT105600.033.67288254
CCGTCTT107500.033.3357857
GCTTGAA100800.033.22847766
TGCCGGC14750.032.03092255
TGCTTGA96500.031.69882465
GTATGCC123050.031.60841452
CGTATGC121000.031.24825351
GATCGCG13400.030.83341446
AGTGATC108050.030.75005543
CACAGTG194000.030.70716340
TCGTATG109300.030.62421850
Produced by FastQC (version 0.11.5)