FastQCFastQC Report
Tue 2 Jul 2019
HLTFCAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFCAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17273975
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA1185760.6864430451010842TruSeq Adapter, Index 14 (97% over 38bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG299390.17331853264810215No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCAGA731200.040.7968671
ATGCCGG168450.038.6430870
GACGTGT2936350.033.7586061
GTGACTG79100.030.7722841
TGACTGG78850.029.8472082
CGCGTAT325300.029.80487669
TTCAGAC1034400.029.4592722
GCGTATG411100.028.68608570
TCGCGTA301950.028.4334968
TACGTGT37550.028.2726841
ACGTGTG3726450.027.1830372
GTATGCC1799450.026.94837470
ATCGCGT331400.026.76304667
GGATGCC131250.026.14945470
GTATGGC106600.026.03378170
GACTGGA95450.025.3872243
GGATGGC45300.025.12547170
TCAGACG1242250.024.9088953
GTTTGCC104850.024.4580570
ACTGGAG98300.024.1186494
Produced by FastQC (version 0.11.5)