FastQCFastQC Report
Tue 2 Jul 2019
HLTFCAFXY_n01_ncm2-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFCAFXY_n01_ncm2-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14197727
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG2156591.518968494041335TruSeq Adapter, Index 12 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATG418940.29507540185833975TruSeq Adapter, Index 12 (97% over 49bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT207060.1458402461182695TruSeq Adapter, Index 12 (100% over 38bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTA167130.11771602595260496TruSeq Adapter, Index 12 (100% over 39bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG143810.10129086155833256No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTG140400.032.03892570
AAAAGGG141500.031.09716270
CGTGTGC243800.031.0817091
TGTGCTC467900.030.4379481
GTGCTCT483850.029.6764432
AAAAAGG352600.029.22788670
GTGTGCT264600.028.7138252
ATGCCGG222100.028.68565470
ACGTGTG121900.027.966361
CTCTTCC1328550.024.640741
TCTTCCG1373400.023.9066892
CTTCCGA1366450.023.8310783
TGAAAAA1018400.023.53166663
TTGAAAA909800.023.33648162
TGCTCTT828650.023.1171881
GCTCTTC849900.023.1125722
TCCGATC1404200.023.075775
GAAAAAA1065450.023.04439564
CCGATCT1403500.022.93266
CTTGAAA910700.022.91378261
Produced by FastQC (version 0.11.5)