FastQCFastQC Report
Tue 2 Jul 2019
HLTFCAFXY_n01_ncm2-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFCAFXY_n01_ncm2-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15278920
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG2305901.5092035300924411TruSeq Adapter, Index 11 (100% over 49bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTA208990.1367832281339257TruSeq Adapter, Index 11 (100% over 38bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTAC165390.10824717977448667TruSeq Adapter, Index 11 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG109500.042.38937470
TGTGCTC397650.035.014571
GTGCTCT409300.034.2186972
CGTGTGC159500.032.2802541
CGTCTTG73100.030.07252570
AAAAGGG151300.027.69381970
GGATCGG30850.027.5699581
GTGTGCT194400.026.4816192
CTCTTCC1268050.025.841471
TGAAAAA828400.025.67194663
TCTTCCG1297650.025.2675882
CTTCCGA1292250.025.134773
TGCTCTT760550.025.0953181
GCTCTTC783100.025.0443082
TTGAAAA814300.024.99909462
CTTGAAA814400.024.79406461
GAAAAAA882250.024.68801564
GCCGTCG140950.024.3878870
TCCGATC1332700.024.2563385
CCGATCT1330800.024.0594736
Produced by FastQC (version 0.11.5)