FastQCFastQC Report
Tue 2 Jul 2019
HLTFCAFXY_n01_ncm2-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFCAFXY_n01_ncm2-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13301362
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG310020.23307387619403186TruSeq Adapter, Index 10 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCTC156800.029.3103331
GTGCTCT161500.028.6472282
CTTCCGA447250.022.9657063
TCCGATC448750.022.7095745
TCTTCCG461250.022.4811732
CCGATCT450200.022.3177176
CGTGTGC90300.021.9784661
CTCTTCC476750.021.8936921
TTCCGAT472250.021.6832524
GTGTGCT96450.020.682182
CGATCTC118400.019.861947
CGATCTG180500.019.8142577
GCTCTTC312200.019.280542
CGGCGTG60800.018.25108770
CGTGAAA98150.018.18691667
GCGTGAA91250.018.14293166
TGCTCTT325950.018.0732041
ACGTGTG45200.017.8885691
AAAAAGG228800.017.59441870
CGATCTA141600.017.0525747
Produced by FastQC (version 0.11.5)