FastQCFastQC Report
Tue 2 Jul 2019
HLTFCAFXY_n01_ncm2-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFCAFXY_n01_ncm2-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13750583
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG600430.4366578493435515TruSeq Adapter, Index 9 (100% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTCT127250.028.6571162
TGTGCTC127350.028.4733561
ATGCCGG28500.026.77681770
TCCGATC347200.022.779795
CTTCCGA351250.022.7064363
CCGATCT347550.022.6360176
CGTGTGC49400.022.105761
TCTTCCG367200.021.9870762
TTCCGAT369750.021.4946294
CTCTTCC389000.020.8385141
CGATCTC101800.019.2161357
CGATCTG144250.018.9225987
GCTCTTC258800.018.2689532
TGCTCTT280900.016.4724251
CGATCTA103000.016.2742257
GCCGTCG32500.015.83366170
GTGTGCT69050.015.7622882
GCTTGAA364250.015.49219170
CTTGAAA371950.015.12893161
GATCTGC95800.014.5750288
Produced by FastQC (version 0.11.5)