FastQCFastQC Report
Tue 2 Jul 2019
HLTFCAFXY_n01_ncm2-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFCAFXY_n01_ncm2-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18089306
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG708280.3915462539027202TruSeq Adapter, Index 7 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCTC397950.028.4790421
ACGTGTG110950.027.6346421
GTGCTCT414500.027.4991862
CGTGTGC236400.027.405391
GTGTGCT241250.026.7642842
CGTCTTG158500.026.037470
CTTCCGA1113500.022.7170623
TCTTCCG1128750.022.5373862
CTCTTCC1143250.022.3153531
TCCGATC1145950.021.9424615
CCGATCT1140700.021.8194856
TTCCGAT1165150.021.6259354
GCTCTTC734400.021.353492
TGCTCTT739100.020.6850451
AAAAAGG317150.020.40728670
CGATCTG434550.020.3182967
CGATCTC282700.020.3138687
CGTCGTG90100.019.85222270
GACGTGT65600.019.7946171
CGGGGTG83850.019.36992670
Produced by FastQC (version 0.11.5)