FastQCFastQC Report
Tue 2 Jul 2019
HLTFCAFXY_n01_ncm2-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFCAFXY_n01_ncm2-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13757834
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG2703741.9652366789714135TruSeq Adapter, Index 4 (100% over 49bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC208100.15125927526091681TruSeq Adapter, Index 4 (100% over 38bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA170620.12401661482468825TruSeq Adapter, Index 4 (100% over 39bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG156290.11360073104530843No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG108950.040.22643770
TGTGCTC373000.036.16461
GTGCTCT387300.035.1237142
AAAAGGG142100.032.4187570
CGTGTGC147000.030.7151871
AAAAAGG360900.029.50575670
CGTCTTG72800.029.37949270
TGAAAAA970300.026.89496863
TTGAAAA940300.026.74072662
CTCTTCC1257150.026.5218751
TGCTCTT733750.026.502991
GCTCTTC755650.026.3387992
TCTTCCG1286650.025.9708022
GTGTGCT174950.025.785312
CTTCCGA1285300.025.744763
GAAAAAA1035550.025.57533664
CTTGAAA989250.025.23005561
TCCGATC1333600.024.7099085
TTCCGAT1343100.024.6133884
CCGATCT1331750.024.5287446
Produced by FastQC (version 0.11.5)