FastQCFastQC Report
Tue 2 Jul 2019
HLTFCAFXY_n01_ncm2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFCAFXY_n01_ncm2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14393803
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG1501601.0432267275021063TruSeq Adapter, Index 3 (100% over 49bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG224510.15597684642481213TruSeq Adapter, Index 3 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATG193130.1341757977374013TruSeq Adapter, Index 3 (97% over 49bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGC188970.13128566508795486TruSeq Adapter, Index 3 (100% over 39bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG161270.11204127220582358No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCTC473750.031.90861
CGTCTTG171900.031.31828570
GTGCTCT492100.030.850572
CGTGTGC250650.030.8462071
GTGTGCT262750.029.4624882
AAAAAGG351450.028.83384370
AAAAGGG137500.028.28322270
ACGTGTG110800.027.8929671
TGTAGGG29400.026.3098721
GTAGGGA30950.026.0072352
CTCTTCC1395000.024.9117981
GCTCTTC875950.024.5150642
TGCTCTT858100.024.3343161
TCTTCCG1439050.024.2317542
CTTCCGA1441200.023.960063
CGTCGTG83050.023.81367370
TGAAAAA824550.023.58554363
TCCGATC1486300.022.9999125
TTCCGAT1503600.022.8609684
TTGAAAA693500.022.84972262
Produced by FastQC (version 0.11.5)