FastQCFastQC Report
Tue 2 Jul 2019
HLTFCAFXY_n01_ncm2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTFCAFXY_n01_ncm2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13764140
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG491600.3571599823890196TruSeq Adapter, Index 1 (100% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC49150.026.419671
ATGCCGG27600.024.09767770
TGTGCTC109800.023.780091
GTGCTCT111950.023.4142952
TCCGATC277950.021.542795
CCGATCT278450.021.365786
CTTCCGA287000.021.1440123
TCTTCCG301250.020.4227942
TTCCGAT305300.019.7390634
GTGTGCT67050.019.2597642
CTCTTCC332800.018.6572361
CGATCTG119850.017.3734047
GCTCTTC225450.016.5784662
GCTTGAA352950.015.86855870
ACGTGTG36050.015.5343031
CTTGAAA369150.015.19879867
GCCGTCG30150.014.977283570
TGCTTGA371750.014.91518269
TGCTCTT244400.014.7936781
CTGCTTG382600.014.39067968
Produced by FastQC (version 0.11.5)