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        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.9

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        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2023-12-21, 16:43 based on data in: /scratch/gencore/logs/html/HLTC7DRX3/merged


        General Statistics

        Showing 274/274 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        HLTC7DRX3_n01_AtTGA5_Fa-B_ag
        29.2%
        42%
        13.1
        HLTC7DRX3_n01_AtTGA5_Pda-B_ag
        35.9%
        49%
        38.2
        HLTC7DRX3_n01_AtbZIP12_An1AmpPoolHm-B_ag1
        31.3%
        40%
        2.9
        HLTC7DRX3_n01_AtbZIP12_An1AmpPoolHm-B_ag2
        32.0%
        40%
        3.3
        HLTC7DRX3_n01_AtbZIP12_C24AmpPoolHm-B_ag1
        34.8%
        40%
        10.1
        HLTC7DRX3_n01_AtbZIP12_C24AmpPoolHm-B_ag2
        34.6%
        39%
        11.1
        HLTC7DRX3_n01_AtbZIP12_ColAmpPoolHm-B_ag1
        26.6%
        40%
        2.5
        HLTC7DRX3_n01_AtbZIP12_ColAmpPoolHm-B_ag2
        26.8%
        40%
        2.7
        HLTC7DRX3_n01_AtbZIP12_CviAmpPoolHm-B_ag1
        36.6%
        40%
        6.1
        HLTC7DRX3_n01_AtbZIP12_CviAmpPoolHm-B_ag2
        37.2%
        39%
        6.9
        HLTC7DRX3_n01_AtbZIP12_EriAmpPoolHm-B_ag1
        28.1%
        39%
        4.8
        HLTC7DRX3_n01_AtbZIP12_EriAmpPoolHm-B_ag2
        28.6%
        39%
        5.5
        HLTC7DRX3_n01_AtbZIP12_KyoAmpPoolHm-B_ag1
        33.7%
        41%
        4.4
        HLTC7DRX3_n01_AtbZIP12_KyoAmpPoolHm-B_ag2
        34.1%
        40%
        5.0
        HLTC7DRX3_n01_AtbZIP12_LerAmpPoolHm-B_ag1
        30.2%
        40%
        3.4
        HLTC7DRX3_n01_AtbZIP12_LerAmpPoolHm-B_ag2
        30.8%
        40%
        3.7
        HLTC7DRX3_n01_AtbZIP12_ShaAmpPoolHm-B_ag1
        30.4%
        39%
        7.0
        HLTC7DRX3_n01_AtbZIP12_ShaAmpPoolHm-B_ag2
        30.3%
        39%
        7.8
        HLTC7DRX3_n01_AtbZIP16_An1AmpPoolHm-B_ag1
        24.1%
        42%
        4.1
        HLTC7DRX3_n01_AtbZIP16_An1AmpPoolHm-B_ag2
        22.7%
        42%
        3.2
        HLTC7DRX3_n01_AtbZIP16_C24AmpPoolHm-B_ag1
        26.7%
        41%
        6.8
        HLTC7DRX3_n01_AtbZIP16_C24AmpPoolHm-B_ag2
        25.7%
        41%
        5.2
        HLTC7DRX3_n01_AtbZIP16_ColAmpPoolHm-B_ag1
        26.4%
        42%
        6.5
        HLTC7DRX3_n01_AtbZIP16_ColAmpPoolHm-B_ag2
        25.4%
        42%
        5.2
        HLTC7DRX3_n01_AtbZIP16_CviAmpPoolHm-B_ag1
        25.7%
        41%
        5.2
        HLTC7DRX3_n01_AtbZIP16_CviAmpPoolHm-B_ag2
        24.5%
        41%
        4.1
        HLTC7DRX3_n01_AtbZIP16_EriAmpPoolHm-B_ag1
        21.4%
        40%
        5.6
        HLTC7DRX3_n01_AtbZIP16_EriAmpPoolHm-B_ag2
        20.4%
        41%
        4.4
        HLTC7DRX3_n01_AtbZIP16_KyoAmpPoolHm-B_ag1
        26.5%
        41%
        4.7
        HLTC7DRX3_n01_AtbZIP16_KyoAmpPoolHm-B_ag2
        25.4%
        42%
        3.6
        HLTC7DRX3_n01_AtbZIP16_LerAmpPoolHm-B_ag1
        25.7%
        42%
        4.3
        HLTC7DRX3_n01_AtbZIP16_LerAmpPoolHm-B_ag2
        24.2%
        42%
        3.3
        HLTC7DRX3_n01_AtbZIP16_ShaAmpPoolHm-B_ag1
        22.6%
        41%
        5.9
        HLTC7DRX3_n01_AtbZIP16_ShaAmpPoolHm-B_ag2
        21.6%
        41%
        4.5
        HLTC7DRX3_n01_AtbZIP18_An1AmpPoolHm-B_ag1
        29.5%
        41%
        5.3
        HLTC7DRX3_n01_AtbZIP18_An1AmpPoolHm-B_ag2
        27.1%
        41%
        3.8
        HLTC7DRX3_n01_AtbZIP18_C24AmpPoolHm-B_ag1
        28.2%
        39%
        5.0
        HLTC7DRX3_n01_AtbZIP18_C24AmpPoolHm-B_ag2
        25.6%
        40%
        3.3
        HLTC7DRX3_n01_AtbZIP18_ColAmpPoolHm-B_ag1
        30.5%
        40%
        5.2
        HLTC7DRX3_n01_AtbZIP18_ColAmpPoolHm-B_ag2
        28.0%
        41%
        3.6
        HLTC7DRX3_n01_AtbZIP18_CviAmpPoolHm-B_ag1
        27.3%
        40%
        3.9
        HLTC7DRX3_n01_AtbZIP18_CviAmpPoolHm-B_ag2
        25.0%
        41%
        2.7
        HLTC7DRX3_n01_AtbZIP18_EriAmpPoolHm-B_ag1
        25.2%
        39%
        4.9
        HLTC7DRX3_n01_AtbZIP18_EriAmpPoolHm-B_ag2
        22.7%
        40%
        3.3
        HLTC7DRX3_n01_AtbZIP18_KyoAmpPoolHm-B_ag1
        29.6%
        40%
        5.0
        HLTC7DRX3_n01_AtbZIP18_KyoAmpPoolHm-B_ag2
        26.9%
        40%
        3.3
        HLTC7DRX3_n01_AtbZIP18_LerAmpPoolHm-B_ag1
        30.7%
        41%
        4.3
        HLTC7DRX3_n01_AtbZIP18_LerAmpPoolHm-B_ag2
        28.2%
        41%
        3.0
        HLTC7DRX3_n01_AtbZIP18_ShaAmpPoolHm-B_ag1
        23.3%
        39%
        4.2
        HLTC7DRX3_n01_AtbZIP18_ShaAmpPoolHm-B_ag2
        20.7%
        40%
        2.9
        HLTC7DRX3_n01_AtbZIP20_An1AmpPoolHm-B_ag1
        27.8%
        38%
        2.9
        HLTC7DRX3_n01_AtbZIP20_An1AmpPoolHm-B_ag2
        26.5%
        39%
        2.4
        HLTC7DRX3_n01_AtbZIP20_C24AmpPoolHm-B_ag1
        29.5%
        38%
        4.6
        HLTC7DRX3_n01_AtbZIP20_C24AmpPoolHm-B_ag2
        28.1%
        38%
        3.9
        HLTC7DRX3_n01_AtbZIP20_ColAmpPoolHm-B_ag1
        30.1%
        38%
        4.4
        HLTC7DRX3_n01_AtbZIP20_ColAmpPoolHm-B_ag2
        28.4%
        39%
        3.6
        HLTC7DRX3_n01_AtbZIP20_CviAmpPoolHm-B_ag1
        28.7%
        38%
        3.2
        HLTC7DRX3_n01_AtbZIP20_CviAmpPoolHm-B_ag2
        27.0%
        39%
        2.7
        HLTC7DRX3_n01_AtbZIP20_EriAmpPoolHm-B_ag1
        25.5%
        37%
        3.8
        HLTC7DRX3_n01_AtbZIP20_EriAmpPoolHm-B_ag2
        23.8%
        38%
        3.1
        HLTC7DRX3_n01_AtbZIP20_KyoAmpPoolHm-B_ag1
        31.1%
        38%
        4.9
        HLTC7DRX3_n01_AtbZIP20_KyoAmpPoolHm-B_ag2
        29.0%
        39%
        3.8
        HLTC7DRX3_n01_AtbZIP20_LerAmpPoolHm-B_ag1
        29.9%
        38%
        3.3
        HLTC7DRX3_n01_AtbZIP20_LerAmpPoolHm-B_ag2
        27.9%
        39%
        2.7
        HLTC7DRX3_n01_AtbZIP20_ShaAmpPoolHm-B_ag1
        27.5%
        38%
        4.3
        HLTC7DRX3_n01_AtbZIP20_ShaAmpPoolHm-B_ag2
        26.3%
        38%
        3.6
        HLTC7DRX3_n01_AtbZIP23_An1AmpPoolHm-B_ag1
        27.5%
        39%
        4.6
        HLTC7DRX3_n01_AtbZIP23_An1AmpPoolHm-B_ag2
        29.9%
        39%
        5.8
        HLTC7DRX3_n01_AtbZIP23_C24AmpPoolHm-B_ag1
        28.2%
        38%
        7.2
        HLTC7DRX3_n01_AtbZIP23_C24AmpPoolHm-B_ag2
        29.5%
        38%
        8.0
        HLTC7DRX3_n01_AtbZIP23_ColAmpPoolHm-B_ag1
        27.8%
        39%
        5.8
        HLTC7DRX3_n01_AtbZIP23_ColAmpPoolHm-B_ag2
        30.0%
        39%
        7.5
        HLTC7DRX3_n01_AtbZIP23_CviAmpPoolHm-B_ag1
        29.2%
        39%
        5.2
        HLTC7DRX3_n01_AtbZIP23_CviAmpPoolHm-B_ag2
        30.4%
        38%
        6.1
        HLTC7DRX3_n01_AtbZIP23_EriAmpPoolHm-B_ag1
        24.3%
        38%
        6.1
        HLTC7DRX3_n01_AtbZIP23_EriAmpPoolHm-B_ag2
        26.1%
        38%
        7.1
        HLTC7DRX3_n01_AtbZIP23_KyoAmpPoolHm-B_ag1
        29.6%
        39%
        6.5
        HLTC7DRX3_n01_AtbZIP23_KyoAmpPoolHm-B_ag2
        31.1%
        39%
        7.7
        HLTC7DRX3_n01_AtbZIP23_LerAmpPoolHm-B_ag1
        28.8%
        39%
        5.0
        HLTC7DRX3_n01_AtbZIP23_LerAmpPoolHm-B_ag2
        30.4%
        39%
        5.9
        HLTC7DRX3_n01_AtbZIP23_ShaAmpPoolHm-B_ag1
        23.4%
        38%
        5.5
        HLTC7DRX3_n01_AtbZIP23_ShaAmpPoolHm-B_ag2
        24.7%
        38%
        6.4
        HLTC7DRX3_n01_AtbZIP26_An1AmpPoolHm-B_ag
        27.8%
        39%
        2.8
        HLTC7DRX3_n01_AtbZIP26_C24AmpPoolHm-B_ag
        29.3%
        38%
        4.5
        HLTC7DRX3_n01_AtbZIP26_ColAmpPoolHm-B_ag
        30.4%
        39%
        4.4
        HLTC7DRX3_n01_AtbZIP26_CviAmpPoolHm-B_ag
        28.2%
        39%
        3.1
        HLTC7DRX3_n01_AtbZIP26_EriAmpPoolHm-B_ag
        25.2%
        38%
        3.6
        HLTC7DRX3_n01_AtbZIP26_KyoAmpPoolHm-B_ag
        30.3%
        39%
        4.5
        HLTC7DRX3_n01_AtbZIP26_LerAmpPoolHm-B_ag
        29.8%
        39%
        3.1
        HLTC7DRX3_n01_AtbZIP26_ShaAmpPoolHm-B_ag
        26.9%
        38%
        4.0
        HLTC7DRX3_n01_AtbZIP2_An1AmpPoolHm-B_ag1
        30.7%
        38%
        3.6
        HLTC7DRX3_n01_AtbZIP2_An1AmpPoolHm-B_ag2
        28.3%
        40%
        4.7
        HLTC7DRX3_n01_AtbZIP2_C24AmpPoolHm-B_ag1
        32.6%
        38%
        6.8
        HLTC7DRX3_n01_AtbZIP2_C24AmpPoolHm-B_ag2
        30.4%
        39%
        9.4
        HLTC7DRX3_n01_AtbZIP2_ColAmpPoolHm-B_ag1
        30.2%
        38%
        4.5
        HLTC7DRX3_n01_AtbZIP2_ColAmpPoolHm-B_ag2
        27.7%
        40%
        5.9
        HLTC7DRX3_n01_AtbZIP2_CviAmpPoolHm-B_ag1
        33.8%
        38%
        4.9
        HLTC7DRX3_n01_AtbZIP2_CviAmpPoolHm-B_ag2
        30.9%
        39%
        6.2
        HLTC7DRX3_n01_AtbZIP2_EriAmpPoolHm-B_ag1
        27.7%
        37%
        4.8
        HLTC7DRX3_n01_AtbZIP2_EriAmpPoolHm-B_ag2
        24.4%
        39%
        6.0
        HLTC7DRX3_n01_AtbZIP2_KyoAmpPoolHm-B_ag1
        33.3%
        38%
        5.6
        HLTC7DRX3_n01_AtbZIP2_KyoAmpPoolHm-B_ag2
        30.8%
        40%
        7.2
        HLTC7DRX3_n01_AtbZIP2_LerAmpPoolHm-B_ag1
        31.5%
        38%
        3.9
        HLTC7DRX3_n01_AtbZIP2_LerAmpPoolHm-B_ag2
        29.4%
        40%
        5.1
        HLTC7DRX3_n01_AtbZIP2_ShaAmpPoolHm-B_ag1
        29.9%
        37%
        6.1
        HLTC7DRX3_n01_AtbZIP2_ShaAmpPoolHm-B_ag2
        26.1%
        39%
        7.0
        HLTC7DRX3_n01_AtbZIP49zipD_ampCol-B_ag1
        23.8%
        39%
        4.9
        HLTC7DRX3_n01_AtbZIP49zipD_ampCol-B_ag2
        20.6%
        39%
        2.9
        HLTC7DRX3_n01_AtbZIP56_An1AmpPoolHm-B_ag1
        26.7%
        41%
        4.0
        HLTC7DRX3_n01_AtbZIP56_An1AmpPoolHm-B_ag2
        26.3%
        41%
        4.1
        HLTC7DRX3_n01_AtbZIP56_C24AmpPoolHm-B_ag1
        31.0%
        40%
        10.8
        HLTC7DRX3_n01_AtbZIP56_C24AmpPoolHm-B_ag2
        30.2%
        40%
        11.1
        HLTC7DRX3_n01_AtbZIP56_ColAmpPoolHm-B_ag1
        26.3%
        41%
        5.2
        HLTC7DRX3_n01_AtbZIP56_ColAmpPoolHm-B_ag2
        26.3%
        41%
        5.5
        HLTC7DRX3_n01_AtbZIP56_CviAmpPoolHm-B_ag1
        29.4%
        40%
        5.3
        HLTC7DRX3_n01_AtbZIP56_CviAmpPoolHm-B_ag2
        28.5%
        41%
        5.4
        HLTC7DRX3_n01_AtbZIP56_EriAmpPoolHm-B_ag1
        23.1%
        40%
        5.6
        HLTC7DRX3_n01_AtbZIP56_EriAmpPoolHm-B_ag2
        22.8%
        40%
        5.6
        HLTC7DRX3_n01_AtbZIP56_KyoAmpPoolHm-B_ag1
        28.8%
        41%
        5.1
        HLTC7DRX3_n01_AtbZIP56_KyoAmpPoolHm-B_ag2
        28.3%
        41%
        5.1
        HLTC7DRX3_n01_AtbZIP56_LerAmpPoolHm-B_ag1
        27.9%
        41%
        4.9
        HLTC7DRX3_n01_AtbZIP56_LerAmpPoolHm-B_ag2
        27.6%
        41%
        4.9
        HLTC7DRX3_n01_AtbZIP56_ShaAmpPoolHm-B_ag1
        25.9%
        40%
        7.5
        HLTC7DRX3_n01_AtbZIP56_ShaAmpPoolHm-B_ag2
        25.2%
        40%
        7.3
        HLTC7DRX3_n01_FaWRKY45_Fa-B_ag1
        38.3%
        40%
        31.1
        HLTC7DRX3_n01_FaWRKY45_Fa-B_ag2
        40.2%
        40%
        33.5
        HLTC7DRX3_n01_pIXHALO_An1AmpPoolHm-B_ag
        27.7%
        39%
        3.2
        HLTC7DRX3_n01_pIXHALO_C24AmpPoolHm-B_ag
        29.2%
        38%
        4.6
        HLTC7DRX3_n01_pIXHALO_ColAmpPoolHm-B_ag
        31.7%
        39%
        5.2
        HLTC7DRX3_n01_pIXHALO_CviAmpPoolHm-B_ag
        27.1%
        39%
        3.2
        HLTC7DRX3_n01_pIXHALO_EriAmpPoolHm-B_ag
        25.3%
        38%
        3.9
        HLTC7DRX3_n01_pIXHALO_Fa-B_ag
        23.9%
        40%
        3.1
        HLTC7DRX3_n01_pIXHALO_KyoAmpPoolHm-B_ag
        30.8%
        39%
        5.1
        HLTC7DRX3_n01_pIXHALO_LerAmpPoolHm-B_ag
        30.1%
        39%
        3.5
        HLTC7DRX3_n01_pIXHALO_Pda-B_ag
        23.9%
        48%
        19.1
        HLTC7DRX3_n01_pIXHALO_ShaAmpPoolHm-B_ag
        24.2%
        38%
        3.3
        HLTC7DRX3_n01_undetermined
        61.3%
        42%
        113.8
        HLTC7DRX3_n02_AtTGA5_Fa-B_ag
        25.8%
        42%
        13.1
        HLTC7DRX3_n02_AtTGA5_Pda-B_ag
        32.7%
        50%
        38.2
        HLTC7DRX3_n02_AtbZIP12_An1AmpPoolHm-B_ag1
        31.0%
        41%
        2.9
        HLTC7DRX3_n02_AtbZIP12_An1AmpPoolHm-B_ag2
        31.1%
        40%
        3.3
        HLTC7DRX3_n02_AtbZIP12_C24AmpPoolHm-B_ag1
        34.5%
        40%
        10.1
        HLTC7DRX3_n02_AtbZIP12_C24AmpPoolHm-B_ag2
        33.6%
        39%
        11.1
        HLTC7DRX3_n02_AtbZIP12_ColAmpPoolHm-B_ag1
        25.6%
        40%
        2.5
        HLTC7DRX3_n02_AtbZIP12_ColAmpPoolHm-B_ag2
        25.7%
        40%
        2.7
        HLTC7DRX3_n02_AtbZIP12_CviAmpPoolHm-B_ag1
        36.0%
        40%
        6.1
        HLTC7DRX3_n02_AtbZIP12_CviAmpPoolHm-B_ag2
        36.2%
        39%
        6.9
        HLTC7DRX3_n02_AtbZIP12_EriAmpPoolHm-B_ag1
        27.8%
        39%
        4.8
        HLTC7DRX3_n02_AtbZIP12_EriAmpPoolHm-B_ag2
        27.8%
        39%
        5.5
        HLTC7DRX3_n02_AtbZIP12_KyoAmpPoolHm-B_ag1
        32.5%
        41%
        4.4
        HLTC7DRX3_n02_AtbZIP12_KyoAmpPoolHm-B_ag2
        32.4%
        40%
        5.0
        HLTC7DRX3_n02_AtbZIP12_LerAmpPoolHm-B_ag1
        29.1%
        41%
        3.4
        HLTC7DRX3_n02_AtbZIP12_LerAmpPoolHm-B_ag2
        29.5%
        40%
        3.7
        HLTC7DRX3_n02_AtbZIP12_ShaAmpPoolHm-B_ag1
        29.4%
        39%
        7.0
        HLTC7DRX3_n02_AtbZIP12_ShaAmpPoolHm-B_ag2
        28.9%
        38%
        7.8
        HLTC7DRX3_n02_AtbZIP16_An1AmpPoolHm-B_ag1
        22.3%
        42%
        4.1
        HLTC7DRX3_n02_AtbZIP16_An1AmpPoolHm-B_ag2
        20.8%
        42%
        3.2
        HLTC7DRX3_n02_AtbZIP16_C24AmpPoolHm-B_ag1
        25.6%
        41%
        6.8
        HLTC7DRX3_n02_AtbZIP16_C24AmpPoolHm-B_ag2
        24.3%
        41%
        5.2
        HLTC7DRX3_n02_AtbZIP16_ColAmpPoolHm-B_ag1
        24.5%
        42%
        6.5
        HLTC7DRX3_n02_AtbZIP16_ColAmpPoolHm-B_ag2
        23.4%
        42%
        5.2
        HLTC7DRX3_n02_AtbZIP16_CviAmpPoolHm-B_ag1
        24.0%
        41%
        5.2
        HLTC7DRX3_n02_AtbZIP16_CviAmpPoolHm-B_ag2
        22.6%
        41%
        4.1
        HLTC7DRX3_n02_AtbZIP16_EriAmpPoolHm-B_ag1
        20.0%
        40%
        5.6
        HLTC7DRX3_n02_AtbZIP16_EriAmpPoolHm-B_ag2
        19.0%
        40%
        4.4
        HLTC7DRX3_n02_AtbZIP16_KyoAmpPoolHm-B_ag1
        24.4%
        41%
        4.7
        HLTC7DRX3_n02_AtbZIP16_KyoAmpPoolHm-B_ag2
        23.3%
        42%
        3.6
        HLTC7DRX3_n02_AtbZIP16_LerAmpPoolHm-B_ag1
        24.0%
        42%
        4.3
        HLTC7DRX3_n02_AtbZIP16_LerAmpPoolHm-B_ag2
        22.4%
        42%
        3.3
        HLTC7DRX3_n02_AtbZIP16_ShaAmpPoolHm-B_ag1
        20.9%
        41%
        5.9
        HLTC7DRX3_n02_AtbZIP16_ShaAmpPoolHm-B_ag2
        19.7%
        41%
        4.5
        HLTC7DRX3_n02_AtbZIP18_An1AmpPoolHm-B_ag1
        26.7%
        41%
        5.3
        HLTC7DRX3_n02_AtbZIP18_An1AmpPoolHm-B_ag2
        24.5%
        41%
        3.8
        HLTC7DRX3_n02_AtbZIP18_C24AmpPoolHm-B_ag1
        26.6%
        39%
        5.0
        HLTC7DRX3_n02_AtbZIP18_C24AmpPoolHm-B_ag2
        24.1%
        40%
        3.3
        HLTC7DRX3_n02_AtbZIP18_ColAmpPoolHm-B_ag1
        27.9%
        40%
        5.2
        HLTC7DRX3_n02_AtbZIP18_ColAmpPoolHm-B_ag2
        25.7%
        41%
        3.6
        HLTC7DRX3_n02_AtbZIP18_CviAmpPoolHm-B_ag1
        25.1%
        40%
        3.9
        HLTC7DRX3_n02_AtbZIP18_CviAmpPoolHm-B_ag2
        22.9%
        41%
        2.7
        HLTC7DRX3_n02_AtbZIP18_EriAmpPoolHm-B_ag1
        23.2%
        39%
        4.9
        HLTC7DRX3_n02_AtbZIP18_EriAmpPoolHm-B_ag2
        20.7%
        40%
        3.3
        HLTC7DRX3_n02_AtbZIP18_KyoAmpPoolHm-B_ag1
        26.9%
        40%
        5.0
        HLTC7DRX3_n02_AtbZIP18_KyoAmpPoolHm-B_ag2
        24.6%
        40%
        3.3
        HLTC7DRX3_n02_AtbZIP18_LerAmpPoolHm-B_ag1
        28.2%
        41%
        4.3
        HLTC7DRX3_n02_AtbZIP18_LerAmpPoolHm-B_ag2
        25.6%
        41%
        3.0
        HLTC7DRX3_n02_AtbZIP18_ShaAmpPoolHm-B_ag1
        21.2%
        39%
        4.2
        HLTC7DRX3_n02_AtbZIP18_ShaAmpPoolHm-B_ag2
        18.9%
        40%
        2.9
        HLTC7DRX3_n02_AtbZIP20_An1AmpPoolHm-B_ag1
        25.8%
        38%
        2.9
        HLTC7DRX3_n02_AtbZIP20_An1AmpPoolHm-B_ag2
        24.8%
        39%
        2.4
        HLTC7DRX3_n02_AtbZIP20_C24AmpPoolHm-B_ag1
        28.1%
        38%
        4.6
        HLTC7DRX3_n02_AtbZIP20_C24AmpPoolHm-B_ag2
        26.9%
        38%
        3.9
        HLTC7DRX3_n02_AtbZIP20_ColAmpPoolHm-B_ag1
        28.1%
        38%
        4.4
        HLTC7DRX3_n02_AtbZIP20_ColAmpPoolHm-B_ag2
        26.7%
        39%
        3.6
        HLTC7DRX3_n02_AtbZIP20_CviAmpPoolHm-B_ag1
        26.8%
        38%
        3.2
        HLTC7DRX3_n02_AtbZIP20_CviAmpPoolHm-B_ag2
        25.8%
        38%
        2.7
        HLTC7DRX3_n02_AtbZIP20_EriAmpPoolHm-B_ag1
        24.1%
        38%
        3.8
        HLTC7DRX3_n02_AtbZIP20_EriAmpPoolHm-B_ag2
        22.5%
        38%
        3.1
        HLTC7DRX3_n02_AtbZIP20_KyoAmpPoolHm-B_ag1
        29.0%
        38%
        4.9
        HLTC7DRX3_n02_AtbZIP20_KyoAmpPoolHm-B_ag2
        27.2%
        39%
        3.8
        HLTC7DRX3_n02_AtbZIP20_LerAmpPoolHm-B_ag1
        28.0%
        38%
        3.3
        HLTC7DRX3_n02_AtbZIP20_LerAmpPoolHm-B_ag2
        26.2%
        39%
        2.7
        HLTC7DRX3_n02_AtbZIP20_ShaAmpPoolHm-B_ag1
        25.5%
        38%
        4.3
        HLTC7DRX3_n02_AtbZIP20_ShaAmpPoolHm-B_ag2
        24.6%
        38%
        3.6
        HLTC7DRX3_n02_AtbZIP23_An1AmpPoolHm-B_ag1
        25.8%
        39%
        4.6
        HLTC7DRX3_n02_AtbZIP23_An1AmpPoolHm-B_ag2
        27.8%
        39%
        5.8
        HLTC7DRX3_n02_AtbZIP23_C24AmpPoolHm-B_ag1
        27.0%
        38%
        7.2
        HLTC7DRX3_n02_AtbZIP23_C24AmpPoolHm-B_ag2
        27.9%
        38%
        8.0
        HLTC7DRX3_n02_AtbZIP23_ColAmpPoolHm-B_ag1
        26.2%
        39%
        5.8
        HLTC7DRX3_n02_AtbZIP23_ColAmpPoolHm-B_ag2
        27.9%
        38%
        7.5
        HLTC7DRX3_n02_AtbZIP23_CviAmpPoolHm-B_ag1
        27.8%
        39%
        5.2
        HLTC7DRX3_n02_AtbZIP23_CviAmpPoolHm-B_ag2
        28.7%
        38%
        6.1
        HLTC7DRX3_n02_AtbZIP23_EriAmpPoolHm-B_ag1
        23.0%
        38%
        6.1
        HLTC7DRX3_n02_AtbZIP23_EriAmpPoolHm-B_ag2
        24.5%
        38%
        7.1
        HLTC7DRX3_n02_AtbZIP23_KyoAmpPoolHm-B_ag1
        27.7%
        39%
        6.5
        HLTC7DRX3_n02_AtbZIP23_KyoAmpPoolHm-B_ag2
        29.1%
        39%
        7.7
        HLTC7DRX3_n02_AtbZIP23_LerAmpPoolHm-B_ag1
        27.1%
        39%
        5.0
        HLTC7DRX3_n02_AtbZIP23_LerAmpPoolHm-B_ag2
        28.3%
        39%
        5.9
        HLTC7DRX3_n02_AtbZIP23_ShaAmpPoolHm-B_ag1
        22.0%
        38%
        5.5
        HLTC7DRX3_n02_AtbZIP23_ShaAmpPoolHm-B_ag2
        23.0%
        38%
        6.4
        HLTC7DRX3_n02_AtbZIP26_An1AmpPoolHm-B_ag
        25.7%
        39%
        2.8
        HLTC7DRX3_n02_AtbZIP26_C24AmpPoolHm-B_ag
        27.9%
        38%
        4.5
        HLTC7DRX3_n02_AtbZIP26_ColAmpPoolHm-B_ag
        28.2%
        39%
        4.4
        HLTC7DRX3_n02_AtbZIP26_CviAmpPoolHm-B_ag
        26.3%
        39%
        3.1
        HLTC7DRX3_n02_AtbZIP26_EriAmpPoolHm-B_ag
        23.5%
        38%
        3.6
        HLTC7DRX3_n02_AtbZIP26_KyoAmpPoolHm-B_ag
        28.3%
        39%
        4.5
        HLTC7DRX3_n02_AtbZIP26_LerAmpPoolHm-B_ag
        27.6%
        39%
        3.1
        HLTC7DRX3_n02_AtbZIP26_ShaAmpPoolHm-B_ag
        24.8%
        38%
        4.0
        HLTC7DRX3_n02_AtbZIP2_An1AmpPoolHm-B_ag1
        28.5%
        39%
        3.6
        HLTC7DRX3_n02_AtbZIP2_An1AmpPoolHm-B_ag2
        26.6%
        40%
        4.7
        HLTC7DRX3_n02_AtbZIP2_C24AmpPoolHm-B_ag1
        31.1%
        38%
        6.8
        HLTC7DRX3_n02_AtbZIP2_C24AmpPoolHm-B_ag2
        29.4%
        39%
        9.4
        HLTC7DRX3_n02_AtbZIP2_ColAmpPoolHm-B_ag1
        28.1%
        38%
        4.5
        HLTC7DRX3_n02_AtbZIP2_ColAmpPoolHm-B_ag2
        26.2%
        40%
        5.9
        HLTC7DRX3_n02_AtbZIP2_CviAmpPoolHm-B_ag1
        32.0%
        38%
        4.9
        HLTC7DRX3_n02_AtbZIP2_CviAmpPoolHm-B_ag2
        29.6%
        39%
        6.2
        HLTC7DRX3_n02_AtbZIP2_EriAmpPoolHm-B_ag1
        26.0%
        38%
        4.8
        HLTC7DRX3_n02_AtbZIP2_EriAmpPoolHm-B_ag2
        23.4%
        39%
        6.0
        HLTC7DRX3_n02_AtbZIP2_KyoAmpPoolHm-B_ag1
        31.2%
        39%
        5.6
        HLTC7DRX3_n02_AtbZIP2_KyoAmpPoolHm-B_ag2
        29.2%
        40%
        7.2
        HLTC7DRX3_n02_AtbZIP2_LerAmpPoolHm-B_ag1
        29.3%
        38%
        3.9
        HLTC7DRX3_n02_AtbZIP2_LerAmpPoolHm-B_ag2
        27.7%
        40%
        5.1
        HLTC7DRX3_n02_AtbZIP2_ShaAmpPoolHm-B_ag1
        27.8%
        37%
        6.1
        HLTC7DRX3_n02_AtbZIP2_ShaAmpPoolHm-B_ag2
        24.6%
        39%
        7.0
        HLTC7DRX3_n02_AtbZIP49zipD_ampCol-B_ag1
        22.1%
        39%
        4.9
        HLTC7DRX3_n02_AtbZIP49zipD_ampCol-B_ag2
        19.3%
        39%
        2.9
        HLTC7DRX3_n02_AtbZIP56_An1AmpPoolHm-B_ag1
        25.9%
        41%
        4.0
        HLTC7DRX3_n02_AtbZIP56_An1AmpPoolHm-B_ag2
        25.5%
        41%
        4.1
        HLTC7DRX3_n02_AtbZIP56_C24AmpPoolHm-B_ag1
        30.2%
        40%
        10.8
        HLTC7DRX3_n02_AtbZIP56_C24AmpPoolHm-B_ag2
        29.4%
        40%
        11.1
        HLTC7DRX3_n02_AtbZIP56_ColAmpPoolHm-B_ag1
        25.1%
        41%
        5.2
        HLTC7DRX3_n02_AtbZIP56_ColAmpPoolHm-B_ag2
        24.9%
        41%
        5.5
        HLTC7DRX3_n02_AtbZIP56_CviAmpPoolHm-B_ag1
        28.5%
        40%
        5.3
        HLTC7DRX3_n02_AtbZIP56_CviAmpPoolHm-B_ag2
        27.5%
        41%
        5.4
        HLTC7DRX3_n02_AtbZIP56_EriAmpPoolHm-B_ag1
        22.4%
        40%
        5.6
        HLTC7DRX3_n02_AtbZIP56_EriAmpPoolHm-B_ag2
        21.8%
        40%
        5.6
        HLTC7DRX3_n02_AtbZIP56_KyoAmpPoolHm-B_ag1
        27.2%
        41%
        5.1
        HLTC7DRX3_n02_AtbZIP56_KyoAmpPoolHm-B_ag2
        26.7%
        41%
        5.1
        HLTC7DRX3_n02_AtbZIP56_LerAmpPoolHm-B_ag1
        26.7%
        41%
        4.9
        HLTC7DRX3_n02_AtbZIP56_LerAmpPoolHm-B_ag2
        26.3%
        41%
        4.9
        HLTC7DRX3_n02_AtbZIP56_ShaAmpPoolHm-B_ag1
        24.9%
        40%
        7.5
        HLTC7DRX3_n02_AtbZIP56_ShaAmpPoolHm-B_ag2
        23.9%
        40%
        7.3
        HLTC7DRX3_n02_FaWRKY45_Fa-B_ag1
        35.1%
        41%
        31.1
        HLTC7DRX3_n02_FaWRKY45_Fa-B_ag2
        36.6%
        40%
        33.5
        HLTC7DRX3_n02_pIXHALO_An1AmpPoolHm-B_ag
        25.5%
        39%
        3.2
        HLTC7DRX3_n02_pIXHALO_C24AmpPoolHm-B_ag
        27.8%
        38%
        4.6
        HLTC7DRX3_n02_pIXHALO_ColAmpPoolHm-B_ag
        29.5%
        39%
        5.2
        HLTC7DRX3_n02_pIXHALO_CviAmpPoolHm-B_ag
        25.4%
        39%
        3.2
        HLTC7DRX3_n02_pIXHALO_EriAmpPoolHm-B_ag
        23.7%
        38%
        3.9
        HLTC7DRX3_n02_pIXHALO_Fa-B_ag
        20.6%
        40%
        3.1
        HLTC7DRX3_n02_pIXHALO_KyoAmpPoolHm-B_ag
        28.7%
        39%
        5.1
        HLTC7DRX3_n02_pIXHALO_LerAmpPoolHm-B_ag
        28.1%
        39%
        3.5
        HLTC7DRX3_n02_pIXHALO_Pda-B_ag
        21.8%
        47%
        19.1
        HLTC7DRX3_n02_pIXHALO_ShaAmpPoolHm-B_ag
        22.5%
        38%
        3.3
        HLTC7DRX3_n02_undetermined
        55.9%
        43%
        113.8

        Demultiplexing Report


        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.

        Showing 137/137 rows and 2/2 columns.
        LibraryTotal Read CountPortion (%)
        undetermined_library
        113813722
        12.7
        AtbZIP12_ColAmpPoolHm-B_ag1
        2477949
        0.3
        AtbZIP12_C24AmpPoolHm-B_ag1
        10069262
        1.1
        AtbZIP12_EriAmpPoolHm-B_ag1
        4797939
        0.5
        AtbZIP12_CviAmpPoolHm-B_ag1
        6078400
        0.7
        AtbZIP12_ShaAmpPoolHm-B_ag1
        7005642
        0.8
        AtbZIP12_KyoAmpPoolHm-B_ag1
        4423235
        0.5
        AtbZIP12_LerAmpPoolHm-B_ag1
        3421688
        0.4
        AtbZIP12_An1AmpPoolHm-B_ag1
        2936073
        0.3
        AtbZIP20_ColAmpPoolHm-B_ag1
        4379374
        0.5
        AtbZIP20_C24AmpPoolHm-B_ag1
        4629033
        0.5
        AtbZIP20_EriAmpPoolHm-B_ag1
        3820705
        0.4
        AtbZIP20_CviAmpPoolHm-B_ag1
        3217020
        0.4
        AtbZIP20_ShaAmpPoolHm-B_ag1
        4266202
        0.5
        AtbZIP20_KyoAmpPoolHm-B_ag1
        4865730
        0.5
        AtbZIP20_LerAmpPoolHm-B_ag1
        3288646
        0.4
        AtbZIP20_An1AmpPoolHm-B_ag1
        2949335
        0.3
        AtbZIP23_ColAmpPoolHm-B_ag1
        5838611
        0.7
        AtbZIP23_C24AmpPoolHm-B_ag1
        7242948
        0.8
        AtbZIP23_EriAmpPoolHm-B_ag1
        6073298
        0.7
        AtbZIP23_CviAmpPoolHm-B_ag1
        5222063
        0.6
        AtbZIP23_ShaAmpPoolHm-B_ag1
        5524221
        0.6
        AtbZIP23_KyoAmpPoolHm-B_ag1
        6489991
        0.7
        AtbZIP23_LerAmpPoolHm-B_ag1
        5001016
        0.6
        AtbZIP23_An1AmpPoolHm-B_ag1
        4607399
        0.5
        AtbZIP16_ColAmpPoolHm-B_ag1
        6547946
        0.7
        AtbZIP16_C24AmpPoolHm-B_ag1
        6779902
        0.8
        AtbZIP16_EriAmpPoolHm-B_ag1
        5647490
        0.6
        AtbZIP16_CviAmpPoolHm-B_ag1
        5198122
        0.6
        AtbZIP16_ShaAmpPoolHm-B_ag1
        5876554
        0.7
        AtbZIP16_KyoAmpPoolHm-B_ag1
        4679152
        0.5
        AtbZIP16_LerAmpPoolHm-B_ag1
        4256762
        0.5
        AtbZIP16_An1AmpPoolHm-B_ag1
        4055795
        0.5
        AtbZIP56_ColAmpPoolHm-B_ag1
        5239864
        0.6
        AtbZIP56_C24AmpPoolHm-B_ag1
        10769849
        1.2
        AtbZIP56_EriAmpPoolHm-B_ag1
        5609083
        0.6
        AtbZIP56_CviAmpPoolHm-B_ag1
        5344034
        0.6
        AtbZIP56_ShaAmpPoolHm-B_ag1
        7461544
        0.8
        AtbZIP56_KyoAmpPoolHm-B_ag1
        5115914
        0.6
        AtbZIP56_LerAmpPoolHm-B_ag1
        4872639
        0.5
        AtbZIP56_An1AmpPoolHm-B_ag1
        4001085
        0.4
        AtbZIP18_ColAmpPoolHm-B_ag1
        5215768
        0.6
        AtbZIP18_C24AmpPoolHm-B_ag1
        5038627
        0.6
        AtbZIP18_EriAmpPoolHm-B_ag1
        4923423
        0.6
        AtbZIP18_CviAmpPoolHm-B_ag1
        3896107
        0.4
        AtbZIP18_ShaAmpPoolHm-B_ag1
        4245054
        0.5
        AtbZIP18_KyoAmpPoolHm-B_ag1
        5004559
        0.6
        AtbZIP18_LerAmpPoolHm-B_ag1
        4335640
        0.5
        AtbZIP18_An1AmpPoolHm-B_ag1
        5257506
        0.6
        AtbZIP2_ColAmpPoolHm-B_ag1
        4524656
        0.5
        AtbZIP2_C24AmpPoolHm-B_ag1
        6782622
        0.8
        AtbZIP2_EriAmpPoolHm-B_ag1
        4818036
        0.5
        AtbZIP2_CviAmpPoolHm-B_ag1
        4932609
        0.6
        AtbZIP2_ShaAmpPoolHm-B_ag1
        6052586
        0.7
        AtbZIP2_KyoAmpPoolHm-B_ag1
        5594401
        0.6
        AtbZIP2_LerAmpPoolHm-B_ag1
        3893641
        0.4
        AtbZIP2_An1AmpPoolHm-B_ag1
        3606911
        0.4
        AtbZIP26_ColAmpPoolHm-B_ag
        4352243
        0.5
        AtbZIP26_C24AmpPoolHm-B_ag
        4531215
        0.5
        AtbZIP26_EriAmpPoolHm-B_ag
        3581702
        0.4
        AtbZIP26_CviAmpPoolHm-B_ag
        3079770
        0.3
        AtbZIP26_ShaAmpPoolHm-B_ag
        4035767
        0.5
        AtbZIP26_KyoAmpPoolHm-B_ag
        4487905
        0.5
        AtbZIP26_LerAmpPoolHm-B_ag
        3087803
        0.3
        AtbZIP26_An1AmpPoolHm-B_ag
        2833223
        0.3
        AtbZIP12_ColAmpPoolHm-B_ag2
        2659295
        0.3
        AtbZIP12_C24AmpPoolHm-B_ag2
        11145529
        1.2
        AtbZIP12_EriAmpPoolHm-B_ag2
        5539233
        0.6
        AtbZIP12_CviAmpPoolHm-B_ag2
        6949950
        0.8
        AtbZIP12_ShaAmpPoolHm-B_ag2
        7848087
        0.9
        AtbZIP12_KyoAmpPoolHm-B_ag2
        4950299
        0.6
        AtbZIP12_LerAmpPoolHm-B_ag2
        3736333
        0.4
        AtbZIP12_An1AmpPoolHm-B_ag2
        3262306
        0.4
        AtbZIP20_ColAmpPoolHm-B_ag2
        3581401
        0.4
        AtbZIP20_C24AmpPoolHm-B_ag2
        3878382
        0.4
        AtbZIP20_EriAmpPoolHm-B_ag2
        3085728
        0.3
        AtbZIP20_CviAmpPoolHm-B_ag2
        2671642
        0.3
        AtbZIP20_ShaAmpPoolHm-B_ag2
        3607139
        0.4
        AtbZIP20_KyoAmpPoolHm-B_ag2
        3811915
        0.4
        AtbZIP20_LerAmpPoolHm-B_ag2
        2665637
        0.3
        AtbZIP20_An1AmpPoolHm-B_ag2
        2426331
        0.3
        AtbZIP23_ColAmpPoolHm-B_ag2
        7473515
        0.8
        AtbZIP23_C24AmpPoolHm-B_ag2
        8006557
        0.9
        AtbZIP23_EriAmpPoolHm-B_ag2
        7129433
        0.8
        AtbZIP23_CviAmpPoolHm-B_ag2
        6076487
        0.7
        AtbZIP23_ShaAmpPoolHm-B_ag2
        6389706
        0.7
        AtbZIP23_KyoAmpPoolHm-B_ag2
        7706326
        0.9
        AtbZIP23_LerAmpPoolHm-B_ag2
        5915364
        0.7
        AtbZIP23_An1AmpPoolHm-B_ag2
        5813666
        0.6
        AtbZIP16_ColAmpPoolHm-B_ag2
        5181113
        0.6
        AtbZIP16_C24AmpPoolHm-B_ag2
        5201333
        0.6
        AtbZIP16_EriAmpPoolHm-B_ag2
        4351473
        0.5
        AtbZIP16_CviAmpPoolHm-B_ag2
        4050309
        0.5
        AtbZIP16_ShaAmpPoolHm-B_ag2
        4505127
        0.5
        AtbZIP16_KyoAmpPoolHm-B_ag2
        3561107
        0.4
        AtbZIP16_LerAmpPoolHm-B_ag2
        3313856
        0.4
        AtbZIP16_An1AmpPoolHm-B_ag2
        3169209
        0.4
        AtbZIP56_ColAmpPoolHm-B_ag2
        5474612
        0.6
        AtbZIP56_C24AmpPoolHm-B_ag2
        11098730
        1.2
        AtbZIP56_EriAmpPoolHm-B_ag2
        5567501
        0.6
        AtbZIP56_CviAmpPoolHm-B_ag2
        5401081
        0.6
        AtbZIP56_ShaAmpPoolHm-B_ag2
        7331264
        0.8
        AtbZIP56_KyoAmpPoolHm-B_ag2
        5120022
        0.6
        AtbZIP56_LerAmpPoolHm-B_ag2
        4915675
        0.5
        AtbZIP56_An1AmpPoolHm-B_ag2
        4067866
        0.5
        AtbZIP18_ColAmpPoolHm-B_ag2
        3619048
        0.4
        AtbZIP18_C24AmpPoolHm-B_ag2
        3279323
        0.4
        AtbZIP18_EriAmpPoolHm-B_ag2
        3347818
        0.4
        AtbZIP18_CviAmpPoolHm-B_ag2
        2685769
        0.3
        AtbZIP18_ShaAmpPoolHm-B_ag2
        2882564
        0.3
        AtbZIP18_KyoAmpPoolHm-B_ag2
        3306168
        0.4
        AtbZIP18_LerAmpPoolHm-B_ag2
        3009694
        0.3
        AtbZIP18_An1AmpPoolHm-B_ag2
        3750750
        0.4
        AtbZIP2_ColAmpPoolHm-B_ag2
        5866613
        0.7
        AtbZIP2_C24AmpPoolHm-B_ag2
        9432213
        1.1
        AtbZIP2_EriAmpPoolHm-B_ag2
        6039956
        0.7
        AtbZIP2_CviAmpPoolHm-B_ag2
        6164868
        0.7
        AtbZIP2_ShaAmpPoolHm-B_ag2
        7018244
        0.8
        AtbZIP2_KyoAmpPoolHm-B_ag2
        7158348
        0.8
        AtbZIP2_LerAmpPoolHm-B_ag2
        5105459
        0.6
        AtbZIP2_An1AmpPoolHm-B_ag2
        4669567
        0.5
        pIXHALO_ColAmpPoolHm-B_ag
        5222125
        0.6
        pIXHALO_C24AmpPoolHm-B_ag
        4588892
        0.5
        pIXHALO_EriAmpPoolHm-B_ag
        3919236
        0.4
        pIXHALO_CviAmpPoolHm-B_ag
        3155838
        0.4
        pIXHALO_ShaAmpPoolHm-B_ag
        3340202
        0.4
        pIXHALO_KyoAmpPoolHm-B_ag
        5109522
        0.6
        pIXHALO_LerAmpPoolHm-B_ag
        3512497
        0.4
        pIXHALO_An1AmpPoolHm-B_ag
        3186026
        0.4
        FaWRKY45_Fa-B_ag1
        31095751
        3.5
        FaWRKY45_Fa-B_ag2
        33528307
        3.7
        AtTGA5_Fa-B_ag
        13077521
        1.5
        pIXHALO_Fa-B_ag
        3096345
        0.3
        AtTGA5_Pda-B_ag
        38173494
        4.3
        pIXHALO_Pda-B_ag
        19125485
        2.1
        AtbZIP49zipD_ampCol-B_ag1
        4929348
        0.6
        AtbZIP49zipD_ampCol-B_ag2
        2936960
        0.3

        Barcodes of Undetermined Reads


        We have determined the barcodes of your undetermined reads (reads containing a barcode that you did not encode in your metadata). Here are the top 20 barcodes belonging to the undetermined reads. The full list is available here. If your libraries are dual indexed, the two indicies are concatenated.

        Showing 20/20 rows and 2/2 columns.
        Barcode Sequence(s)CountFrequency (%)
        GGGGGGGGAGATCTCG
        64844542.0
        57.0
        GGGGGGGGCGATCTCG
        1973871.0
        1.7
        GGGGGGGGTAAGATTA
        772320.0
        0.7
        GGGGGGGGCGTGTGAT
        689131.0
        0.6
        GGGGGGGGAGGCTATA
        614077.0
        0.5
        GGGGGGGGCTTCGCCT
        480017.0
        0.4
        GGGGGGGGAAGGCGTA
        453556.0
        0.4
        GGGGGGGGAGTTCTCG
        414037.0
        0.4
        GGGGGGGGCTCGTTCT
        401993.0
        0.3
        GGGGGGGGAGGATAGG
        313154.0
        0.3
        GGGGGGGGAGTCGAAG
        297843.0
        0.3
        GGGGGGGGACGTCCTG
        281910.0
        0.2
        GGGGGGGGAGCTCTCG
        251902.0
        0.2
        GGGGGGGGGTCAGTAC
        235049.0
        0.2
        ATCGATCGGGGGGGGG
        233989.0
        0.2
        GGGGGGGGTAACGTCG
        208967.0
        0.2
        GGGGGGGGAACAGGTG
        207207.0
        0.2
        TACGCTACGGGGGGGG
        198354.0
        0.2
        TGGACTCTGGGGGGGG
        193751.0
        0.2
        CGGCTAATGGGGGGGG
        187983.0
        0.2

        Run Statistics

        Showing 1/1 rows and 4/4 columns.
        Number of LanesTotal # of Single-End ReadsTotal # PF Reads% Undetermined% PhiX Aligned
        2.0
        1276674048
        895981501
        12.7
        7.5

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Counts

        Sequence counts for each sample. Duplicate read counts are an estimate only.

        This plot show the total number of reads, broken down into unique and duplicate if possible (only more recent versions of FastQC give duplicate info).

        You can read more about duplicate calculation in the FastQC documentation. A small part has been copied here for convenience:

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Sequence Quality Histograms

        The mean quality value across each base position in the read.

        To enable multiple samples to be plotted on the same graph, only the mean quality scores are plotted (unlike the box plots seen in FastQC reports).

        Taken from the FastQC help:

        The y-axis on the graph shows the quality scores. The higher the score, the better the base call. The background of the graph divides the y axis into very good quality calls (green), calls of reasonable quality (orange), and calls of poor quality (red). The quality of calls on most platforms will degrade as the run progresses, so it is common to see base calls falling into the orange area towards the end of a read.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality.

        From the FastQC help:

        The per sequence quality score report allows you to see if a subset of your sequences have universally low quality values. It is often the case that a subset of sequences will have universally poor quality, however these should represent only a small percentage of the total sequences.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called.

        To enable multiple samples to be shown in a single plot, the base composition data is shown as a heatmap. The colours represent the balance between the four bases: an even distribution should give an even muddy brown colour. Hover over the plot to see the percentage of the four bases under the cursor.

        To see the data as a line plot, as in the original FastQC graph, click on a sample track.

        From the FastQC help:

        Per Base Sequence Content plots out the proportion of each base position in a file for which each of the four normal DNA bases has been called.

        In a random library you would expect that there would be little to no difference between the different bases of a sequence run, so the lines in this plot should run parallel with each other. The relative amount of each base should reflect the overall amount of these bases in your genome, but in any case they should not be hugely imbalanced from each other.

        It's worth noting that some types of library will always produce biased sequence composition, normally at the start of the read. Libraries produced by priming using random hexamers (including nearly all RNA-Seq libraries) and those which were fragmented using transposases inherit an intrinsic bias in the positions at which reads start. This bias does not concern an absolute sequence, but instead provides enrichement of a number of different K-mers at the 5' end of the reads. Whilst this is a true technical bias, it isn't something which can be corrected by trimming and in most cases doesn't seem to adversely affect the downstream analysis.

        Click a sample row to see a line plot for that dataset.
        Rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content.

        From the FastQC help:

        This module measures the GC content across the whole length of each sequence in a file and compares it to a modelled normal distribution of GC content.

        In a normal random library you would expect to see a roughly normal distribution of GC content where the central peak corresponds to the overall GC content of the underlying genome. Since we don't know the the GC content of the genome the modal GC content is calculated from the observed data and used to build a reference distribution.

        An unusually shaped distribution could indicate a contaminated library or some other kinds of biased subset. A normal distribution which is shifted indicates some systematic bias which is independent of base position. If there is a systematic bias which creates a shifted normal distribution then this won't be flagged as an error by the module since it doesn't know what your genome's GC content should be.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Base N Content

        The percentage of base calls at each position for which an N was called.

        From the FastQC help:

        If a sequencer is unable to make a base call with sufficient confidence then it will normally substitute an N rather than a conventional base call. This graph shows the percentage of base calls at each position for which an N was called.

        It's not unusual to see a very low proportion of Ns appearing in a sequence, especially nearer the end of a sequence. However, if this proportion rises above a few percent it suggests that the analysis pipeline was unable to interpret the data well enough to make valid base calls.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Sequence Length Distribution

        All samples have sequences of a single length (101bp).

        Sequence Duplication Levels

        The relative level of duplication found for every sequence.

        From the FastQC Help:

        In a diverse library most sequences will occur only once in the final set. A low level of duplication may indicate a very high level of coverage of the target sequence, but a high level of duplication is more likely to indicate some kind of enrichment bias (eg PCR over amplification). This graph shows the degree of duplication for every sequence in a library: the relative number of sequences with different degrees of duplication.

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        In a properly diverse library most sequences should fall into the far left of the plot in both the red and blue lines. A general level of enrichment, indicating broad oversequencing in the library will tend to flatten the lines, lowering the low end and generally raising other categories. More specific enrichments of subsets, or the presence of low complexity contaminants will tend to produce spikes towards the right of the plot.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Overrepresented sequences

        The total amount of overrepresented sequences found in each library.

        FastQC calculates and lists overrepresented sequences in FastQ files. It would not be possible to show this for all samples in a MultiQC report, so instead this plot shows the number of sequences categorized as over represented.

        Sometimes, a single sequence may account for a large number of reads in a dataset. To show this, the bars are split into two: the first shows the overrepresented reads that come from the single most common sequence. The second shows the total count from all remaining overrepresented sequences.

        From the FastQC Help:

        A normal high-throughput library will contain a diverse set of sequences, with no individual sequence making up a tiny fraction of the whole. Finding that a single sequence is very overrepresented in the set either means that it is highly biologically significant, or indicates that the library is contaminated, or not as diverse as you expected.

        FastQC lists all of the sequences which make up more than 0.1% of the total. To conserve memory only sequences which appear in the first 100,000 sequences are tracked to the end of the file. It is therefore possible that a sequence which is overrepresented but doesn't appear at the start of the file for some reason could be missed by this module.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position.

        Note that only samples with ≥ 0.1% adapter contamination are shown.

        There may be several lines per sample, as one is shown for each adapter detected in the file.

        From the FastQC Help:

        The plot shows a cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. Once a sequence has been seen in a read it is counted as being present right through to the end of the read so the percentages you see will only increase as the read length goes on.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Status Checks

        Status for each FastQC section showing whether results seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        FastQC assigns a status for each section of the report. These give a quick evaluation of whether the results of the analysis seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        It is important to stress that although the analysis results appear to give a pass/fail result, these evaluations must be taken in the context of what you expect from your library. A 'normal' sample as far as FastQC is concerned is random and diverse. Some experiments may be expected to produce libraries which are biased in particular ways. You should treat the summary evaluations therefore as pointers to where you should concentrate your attention and understand why your library may not look random and diverse.

        Specific guidance on how to interpret the output of each module can be found in the relevant report section, or in the FastQC help.

        In this heatmap, we summarise all of these into a single heatmap for a quick overview. Note that not all FastQC sections have plots in MultiQC reports, but all status checks are shown in this heatmap.

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