FastQCFastQC Report
Thu 21 Dec 2023
HLTC7DRX3_n01_pIXHALO_Pda-B_ag.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTC7DRX3_n01_pIXHALO_Pda-B_ag.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19125485
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT1078850.5640902701290974TruSeq Adapter, Index 7 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATGCC844220.44141102826934847TruSeq Adapter, Index 7 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCGCGTAT515060.2693055888517337TruSeq Adapter, Index 7 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCGCGTATGCC450350.23547115275769476TruSeq Adapter, Index 7 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)