FastQCFastQC Report
Thu 21 Dec 2023
HLTC7DRX3_n01_FaWRKY45_Fa-B_ag2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTC7DRX3_n01_FaWRKY45_Fa-B_ag2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33528307
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT3649031.0883430529313634TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCGCGTAT1911640.5701570317880947TruSeq Adapter, Index 1 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATGCC1421990.4241162549603235TruSeq Adapter, Index 1 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCGCGTATGCC836340.24944295576868822TruSeq Adapter, Index 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)