FastQCFastQC Report
Thu 21 Dec 2023
HLTC7DRX3_n01_AtTGA5_Pda-B_ag.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTC7DRX3_n01_AtTGA5_Pda-B_ag.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38173494
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT4090421.0715340859288385TruSeq Adapter, Index 7 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATGCC3015700.7899984214177513TruSeq Adapter, Index 7 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCGCGTAT1916500.502049930247412TruSeq Adapter, Index 7 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCGCGTATGCC1566660.4104051884797341TruSeq Adapter, Index 7 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGGAT423020.11081511165836692TruSeq Adapter, Index 7 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)