FastQCFastQC Report
Thu 21 Dec 2023
HLTC7DRX3_n01_AtTGA5_Fa-B_ag.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTC7DRX3_n01_AtTGA5_Fa-B_ag.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13077521
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT1796921.374052467589232TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCGCGTAT823950.6300506036274001TruSeq Adapter, Index 1 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATGCC699190.5346502597854746TruSeq Adapter, Index 1 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCGCGTATGCC344200.2631997302852735TruSeq Adapter, Index 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)