FastQCFastQC Report
Wed 7 Dec 2022
HLTC3DRX2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTC3DRX2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1915551440
Sequences flagged as poor quality0
Sequence length24
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCTCGGTGGTCGCCGTATCAT463171762.417955218159007Illumina Single End PCR Primer 1 (100% over 24bp)
CGATCTCGGTGGTCGCCGTATCAT111115150.5800687346720379Illumina Single End PCR Primer 1 (95% over 24bp)
TGATCTCGGTGGTCGCCGTATCAT41679840.21758663917686283Illumina Single End PCR Primer 1 (95% over 24bp)
CGGTCTCGGTGGTCGCCGTATCAT21633110.11293411154753433Illumina Single End PCR Primer 1 (100% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)