FastQCFastQC Report
Wed 7 Dec 2022
HLTC3DRX2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLTC3DRX2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1915551440
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATCAT1472606677.687638343974725No Hit
TAACGTCC1320884456.8955832895826585No Hit
TGTGCCGT1318429576.882767763208698No Hit
GAGTGCGT1271186026.636136171837808No Hit
CTCCAACA1204395936.287463259143801No Hit
TTTCGCGA1172102196.118876087190851No Hit
TGTGTGAC1147920245.99263593777466Illumina PCR Primer Index 5 (100% over 8bp)
ACAGCAAC1141799185.960681379561386No Hit
ATGATACG1120324855.848576167706569Illumina Single End PCR Primer 1 (100% over 8bp)
GACTGTTC1117465955.833651483668849No Hit
AGGTCATA952512404.9725232124280625No Hit
CCACAGAA950816334.963668999669359No Hit
ACAACTTG929397344.851852686347071No Hit
CGCAATTT887924184.635345005404814No Hit
GAGTTGCG884814444.619110828994496No Hit
GGGGGGGG825061564.307175170404194No Hit
GCTGAGGG455506792.3779407876407643No Hit
GCGGAGGG52249340.2727639619012267No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)