FastQCFastQC Report
Wed 25 May 2022
HLNY5AFX3_n01_ColSPL11_Colamp-B_y2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNY5AFX3_n01_ColSPL11_Colamp-B_y2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2708922
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC217880.8043051811753901TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT96820.357411545995049TruSeq Adapter, Index 27 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTTTGCC72590.26796637186305106TruSeq Adapter, Index 27 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGGC59660.22023520795356974TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTTT59460.2194969068876845TruSeq Adapter, Index 27 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTTTGGC52670.19443158570088026TruSeq Adapter, Index 27 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATTCC36620.13518292516358907TruSeq Adapter, Index 27 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCGCGTATGCC33390.12325936294954229TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGGTT28730.106056948114416TruSeq Adapter, Index 27 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)