FastQCFastQC Report
Wed 25 May 2022
HLNY5AFX3_n01_C24SPL11_Colamp-B_y2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNY5AFX3_n01_C24SPL11_Colamp-B_y2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2394517
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC228850.955725100302065TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT101870.42543026422447616TruSeq Adapter, Index 27 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTTTGCC73550.30716006610101326TruSeq Adapter, Index 27 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGGC63850.2666508527607029TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTTT59980.2504889295001873TruSeq Adapter, Index 27 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTTTGGC50700.21173372333543675TruSeq Adapter, Index 27 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATTCC40670.16984636150004362TruSeq Adapter, Index 27 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCGCGTATGCC36780.15360091408831092TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGGTT30990.1294206723109504TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCGCGGTT29210.1219870228526254TruSeq Adapter, Index 27 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGGATGCC28110.11739319453568298TruSeq Adapter, Index 27 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCGCGGTTGGC28090.11730967038446585TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCGCGTAT27390.11438632509186612TruSeq Adapter, Index 27 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTTTTGC26750.11171355225291782TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGGAT26470.11054421413587792TruSeq Adapter, Index 27 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGGTTGGC26090.10895725526275236TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCGCGTTT26070.10887373111153523TruSeq Adapter, Index 27 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATTGC24610.10277646807268438TruSeq Adapter, Index 27 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)