FastQCFastQC Report
Wed 25 May 2022
HLNY5AFX3_n01_C24SPL11_Colamp-B_y1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNY5AFX3_n01_C24SPL11_Colamp-B_y1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2338831
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC250011.068952823012864TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT110300.4716031213884201TruSeq Adapter, Index 27 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTTTGCC76320.32631686513476177TruSeq Adapter, Index 27 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGGC64930.27761732250000104TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTTT61400.26252431235946505TruSeq Adapter, Index 27 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTTTGGC50130.21433784655667726TruSeq Adapter, Index 27 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATTCC44710.19116387631256812TruSeq Adapter, Index 27 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCGCGTATGCC38680.1653817655059301TruSeq Adapter, Index 27 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGGATGCC30850.13190350221969865TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGGTT30720.13134766898506134TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCGCGGTT28970.12386529851878994TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGGAT28660.1225398500361933TruSeq Adapter, Index 27 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCGCGTAT28320.12108613234560342TruSeq Adapter, Index 27 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCGCGGTTGGC27020.1155277999992304TruSeq Adapter, Index 27 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATTGC26660.1139885695033117TruSeq Adapter, Index 27 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGGTTGGC26340.11262036461805064TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCGCGTTT25990.11112389052479636TruSeq Adapter, Index 27 (97% over 39bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTTTTGC25810.11035427527683701TruSeq Adapter, Index 27 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTTTTCC25390.10855850636493189TruSeq Adapter, Index 27 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)