FastQCFastQC Report
Fri 4 Nov 2016
HLNWTBGXY_n01_chxtest_r17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNWTBGXY_n01_chxtest_r17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13167217
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT220100.16715757019877472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA19750.017.80757710
CCGGGGT10150.016.3140981
TCCCTAC23750.014.5180459
GGATAAC116950.013.8934011
GCGGGCT10300.012.727241
CTACACG28300.012.30571212
CCTACAC29950.012.08827211
CACGAGT131600.011.79187368
ACATGGC138200.011.6783246
CATGGCC135500.011.4764447
GGCCATC138050.011.31445310
CATGCGC137150.011.13932428
GATAACA149200.011.0025252
GGCCACG138450.010.98457765
ATGGCCA143950.010.8985788
TACACGA30150.010.8645413
CCGGCAT20750.010.8064521
CGCGGGG10250.010.7699581
TGGCCAT146250.010.7507989
ATGCGCT142300.010.73740329
Produced by FastQC (version 0.11.2)