FastQCFastQC Report
Fri 4 Nov 2016
HLNWTBGXY_n01_chxtest_r15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNWTBGXY_n01_chxtest_r15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12480365
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT242430.1942491265279501No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGA145670.1167193427435816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAAC133500.014.4717691
CCCTACA16100.014.34883510
TCCCTAC17700.013.6361729
CACGAGT147450.012.37203868
ACATGGC161100.012.0086026
CATGGCC155750.011.9766457
GGCCACG154700.011.72549165
GATAACA167450.011.5739942
GGGCGCT4207.669587E-811.4998871
ATGGCCA161850.011.4826488
GGCCATC161800.011.44362210
GGGGCAC7700.011.201191
GCAAGGG49150.011.1606241
TGGCCAT166400.011.1479929
ATAACAT172600.011.1285743
GCCACGA165300.011.11957566
CATGCGC166350.011.06998528
CCGGGGT11000.010.9771651
TAACATG176800.010.8642084
TCATGCG170050.010.84809727
Produced by FastQC (version 0.11.2)