FastQCFastQC Report
Fri 4 Nov 2016
HLNWTBGXY_n01_chxtest_r13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNWTBGXY_n01_chxtest_r13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14170396
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT329940.2328375297345254No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGA177490.12525408605377014No Hit
CTTGACCTCAGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCTGCTTGATCTCGCCCTTCAGGGCGCCGTC149060.10519113227322652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA19450.015.42262410
GGATAAC165800.015.1702551
CCGGGGT11250.014.1076651
GATAACA190900.013.2047382
TCCCTAC23100.012.8364539
ATAACAT193250.012.7761843
ACATGGC194600.012.6521566
CTACACG22450.012.59386812
CACGAGT189900.012.40320868
ATGGCCA197400.012.2791068
CATGGCC196800.012.2289437
TAACATG202600.012.1865634
CGCGGGG9000.011.8841741
GGCCATC201700.011.81219910
CGTATGC17000.011.78711246
TGGCCAT204750.011.720449
GGCCACG200750.011.68172565
CGTTCAC63800.011.67571966
GCCACGA206300.011.53454466
ACGAGTT204400.011.489264569
Produced by FastQC (version 0.11.2)