FastQCFastQC Report
Fri 4 Nov 2016
HLNWTBGXY_n01_chxtest_r12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNWTBGXY_n01_chxtest_r12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18848369
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCA681470.36155382993616053No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT482140.25579932141608647No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT464440.2464085884566458No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC381240.20226683804842743No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGGCCGTTCACGGA339930.18034982231088537No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACTGT292980.15544050522355543No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTC291290.1545438759183885No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGATGTCCCAGGCGA282050.14964159498362964No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCT259390.1376193345960067No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTTCAAGTAGTCGGGGATGTCGGCGGG236020.1252203837902367No Hit
CCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGG230470.1222758319300731No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCC222940.11828079129817545No Hit
GCTTGATGTCGGTCTTGTAGGCGCCGGGCAGCTGCACGGGCTTCTTGGCCATGTAGGTGGTCTTGACCTCGGCGT214390.11374458978386935No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCTTGATCTCGCCCTTCAGGGCGCCGTC206890.10976546564851314No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGG205380.10896433532259475No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAGCGCGCCGAGGGCC197500.10478360223104716No Hit
GTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTC197250.10465096475986863No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTC190160.1008893660772452No Hit
GGGAGGCCTCCACCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGATGAGGCTGAAGCTGA189140.10034820519483675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCAG105400.028.5910158
GCTGACT105700.028.3612121
CTGACTC107250.027.6514992
ACTCTAG116300.026.3294335
GACTCTA121300.025.727344
TGACTCT135250.022.716823
TAGCAGA142150.021.490519
CTCTAGC151050.020.5690166
CTATCGA147200.020.1921417
TCTATCG151250.019.51457216
GATCTAT169750.017.50944314
ATCTATC169250.017.500115
GTGCGCA203100.017.04741768
GCAGATC176750.017.0105411
TGCGCAT205350.016.91092169
AGATCTA178100.016.78497913
TATCGAT178600.016.738718
GGTGCGC209550.016.70398367
AGCAGAT183800.016.50814210
TAGCATG177650.016.47826827
Produced by FastQC (version 0.11.2)