FastQCFastQC Report
Fri 4 Nov 2016
HLNWTBGXY_n01_chxtest_r11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNWTBGXY_n01_chxtest_r11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8983132
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT138030.15365464962554262No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA91520.10187983433840224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGGT6400.018.3283921
TATGCCG10800.016.31434646
CGTATGC12700.015.5067644
GTATGCC12150.015.355535545
GGATAAC74700.014.2712921
GGCCATC83150.012.14954410
ACATGGC86800.012.0381486
GGGCGCC49900.011.81801166
CATGGCC86200.011.7997227
AGGGCGC49450.011.78614265
CCCTACA11450.011.74393610
CAAGGGC28100.011.6592
GATAACA94950.011.5135262
ATGCCGT16600.011.44542347
ATGGCCA88900.011.441358
CGCGGGG6750.011.2446071
TGGCCAT89100.011.2221199
CACGAGT90250.011.15770468
ATAACAT97150.011.1460563
TGCCGTC20150.011.13998948
Produced by FastQC (version 0.11.2)