FastQCFastQC Report
Fri 4 Nov 2016
HLNWTBGXY_n01_chxtest_r09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNWTBGXY_n01_chxtest_r09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14383379
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA231360.16085232823246887No Hit
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT230390.16017793871662564No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154500.10741564968843552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACA19000.015.24354910
GGATAAC127650.014.7577691
TCCCTAC24300.012.7701699
CATGGCC146050.012.2289347
ACATGGC154350.012.17608456
GGCCATC148250.012.07071710
CACGAGT147100.011.95666368
GGCCACG149650.011.68399465
GATAACA162600.011.6648212
ATGGCCA150550.011.6572878
CATGCGC149800.011.53319828
TGGCCAT156900.011.4711779
TAACATG161550.011.4199614
ATGCGCT152650.011.32019329
GCCACGA158850.011.15927566
AACGGCC158800.011.14227162
ATAACAT167150.011.1198833
TCATGCG155600.011.10198427
CGGGCAT12750.011.0948781
GGGCTCC157400.010.9799152
Produced by FastQC (version 0.11.2)