FastQCFastQC Report
Fri 4 Nov 2016
HLNWTBGXY_n01_chxtest_r07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNWTBGXY_n01_chxtest_r07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15743839
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT396890.25209226288454806No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGA212720.13511317030109365No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA207100.1315435199762904No Hit
CTTGACCTCAGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCTGCTTGATCTCGCCCTTCAGGGCGCCGTC198450.12604930728775873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC21600.016.15327844
GGATAAC191550.015.9947331
TATGCCG22950.015.35253546
CCGGGGT14000.015.2795231
CCCTACA21600.014.04817310
GTATGCC25650.013.87185745
GATAACA235150.013.0090872
ACATGGC234300.012.9677176
ATAACAT236350.012.8551193
CATGGCC233450.012.7172937
CACGAGT231850.012.68821368
ATGGCCA242600.012.3371368
TAACATG245600.012.2726634
TCCCTAC27000.012.0048039
ACGAGTT245650.012.00304269
GGCCATC246800.011.97365210
TGGCCAT248500.011.8917399
CGTTCAC76950.011.87677566
GGCCACG246650.011.87105365
GGCCGTT80800.011.69601163
Produced by FastQC (version 0.11.2)