FastQCFastQC Report
Fri 4 Nov 2016
HLNWTBGXY_n01_chxtest_r05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNWTBGXY_n01_chxtest_r05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14793350
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT229780.15532654875332497No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA177460.11995930603953804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAAC126950.014.4854761
CGCGGGG10850.013.0374431
ACATGGC151300.011.9429156
GGCCATC145850.011.86742810
CATGGCC146850.011.8335717
ATGGCCA149950.011.5659378
GATAACA160850.011.4483812
TGGCCAT153100.011.3955329
AGGGCGC88500.011.33954465
GGGCGCC89850.011.28419966
CCGGGGT12550.011.2714141
CACGAGT155000.011.03529968
TAACATG161300.011.0099414
GGCCACG156200.010.99497465
ATAACAT166300.010.8033663
CATGCGC156750.010.78002528
CAGGGCG95300.010.60348264
CGGGGGT12750.010.5534081
CGGGCAT12450.010.5305851
TCATGCG162050.010.5111227
Produced by FastQC (version 0.11.2)