FastQCFastQC Report
Fri 4 Nov 2016
HLNWTBGXY_n01_chxtest_r03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNWTBGXY_n01_chxtest_r03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20700263
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT318590.15390625713306155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAAC174750.014.1557611
GGGCGCC117550.012.17543966
AGGGCGC117200.012.15294665
ACATGGC212500.011.6033166
CCCTACA26250.011.42789410
CATGGCC205000.011.4206867
CAGGGCG126950.011.19257464
GATAACA223000.011.1809832
ATGGCCA214650.010.9875668
GGCCATC211200.010.92215810
GCGCCGT137400.010.69233668
TGGCCGC143000.010.68240720
CACGAGT215400.010.66297168
ATAACAT233300.010.6278393
GCAAGGG75400.010.5699241
AGTGGCC142350.010.5617718
CATGCGC218200.010.47824628
TAACATG235800.010.4566354
TGGCCAT223950.010.4389049
GGCCACG217400.010.34301765
Produced by FastQC (version 0.11.2)