FastQCFastQC Report
Fri 4 Nov 2016
HLNWTBGXY_n01_chxtest_r01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNWTBGXY_n01_chxtest_r01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7601303
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT165280.21743640531103678No Hit
CTTGACCTCAGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCTGCTTGATCTCGCCCTTCAGGGCGCCGTC107180.14100214134339864No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGA80510.1059160514980129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAAC79050.015.4931741
GGGGCCC2404.0282157E-614.36868269
CGTATGC8700.014.29563944
GCAAGGG33850.013.7590691
GCGGGCT6150.013.4632541
TATGCCG9450.013.16054346
CAAGGGC35000.013.104412
GATAACA99200.012.7234032
CTTCCGA33600.012.4239581
GGGCGCC63700.012.39721766
GCGGGAT11200.012.3212811
ACATGGC99750.012.2382286
GGGGCAT7650.012.1763261
ATAACAT100800.012.1447963
AGGGCGC65150.012.0683765
CATGGCC98050.011.9916987
GCGCCGT67900.011.73210968
ATGGCCA101150.011.5900938
TGGCCAT103450.011.4324039
GGCCATC102900.011.42656610
Produced by FastQC (version 0.11.2)