FastQCFastQC Report
Fri 4 Nov 2016
HLNWTBGXY_n01_chxtest_g6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLNWTBGXY_n01_chxtest_g6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16777842
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT62700.021.2929691
CTAGCAG69200.018.5844448
TATGCCG29950.018.56477248
CGTATGC30200.018.53451746
ACTCTAG73900.018.338055
GACTCTA79250.017.8833524
GTATGCC32200.017.48794647
CTTCCGA99100.017.0923041
CTGACTC82250.016.0995832
CCGATCT104700.015.7759384
CTATCGA81900.015.49331817
CGCGGGG11750.014.3863621
CGGGGGT12850.014.228711
TCTATCG89300.014.17052716
TCGTATG40250.014.07921445
CTCTAGC102800.013.417716
CTCGTAT40950.013.41691844
TAGCAGA98750.013.2327539
GCACACG50600.013.08274311
TTCCGAT132000.012.9054262
Produced by FastQC (version 0.11.2)